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CAS RN: | Product Number: A2584

Anti-Sialyl Lewis A Monoclonal Antibody (1H4)

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Product Information
Volume/Quantity: 0.1 mg/vial
Product Form: Purified mouse monoclonal antibody/IgG3, κ-chain
Buffer: Phosphate buffered-saline(-) (PBS(-))
Preparation: The antibody is purified from ascites fluid.
Preservative: 0.05% sodium azide
Stabilizer: None
Immunogen: Immunized with human colon cancer cells (Colo201), and screened using sialyl Lewis A-active glycolipids
Specificity: Sialyl Lewis A
Clone Name: 1H4

Immunohistochemistry,flow-cytometric analysis, ELISA, TLC-immunostaining, Western blotting, cell adhesion inhibition experiments.

Store in freezer (-20゚C to -80゚C). It is not recommended storing at 4゚C. Avoid repeated freezing and thawing.

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Product Number A2584
Physical State (20 deg.C) Liquid
Storage Temperature -80°C
Condition to Avoid Heat Sensitive
Appearance Colorless to Almost colorless clear liquid
Titer(ELISA) to pass test
Properties (reference)
Related Laws:
Transport Information:
HS Number 3002150000
Anti-Sialyl Lewis A Monoclonal Antibody (1H4)

Adhesion of human cancer cells to vascular endothelium mediated by a carbohydrate antigen, sialyl Lewis A

A. Takada, K. Ohmori, N. Takahashi, K. Tsuyuoka, A. Yago, K. Zenita, A. Hasegawa, R. Kannagi, Biochem. Biophys. Res. Commun. 1991, 179, 713-719.

Northern hybridization analysis of VH gene expression in murine monoclonal antibodies directed to cancer-associated ganglioside antigens having various sialic acid linkages

K. Zenita, K. Hirashima, K. Shigeta, N. Hiraiwa, A. Takada, K. Hashimoto, E. Fujimoto, K. Yago, R. Kannagi, J. Immunol. 1990, 144, 4442-4451.

Contribution of carbohydrate antigens sialyl Lewis A and sialyl Lewis X to adhesion of human cancer cells to vascular endothelium

A. Takada, K. Ohmori, T. Yoneda, K. Tsuyuoka, A. Hasegawa, M. Kiso, R. Kannagi, Cancer Res. 1993, 53, 354-361.

Synthesis of disialyl Lewis a (Lea) structure in colon cancer cell lines by a sialyltransferase, ST6GalNAc VI, responsible for the synthesis of α-series gangliosides

A. Tsuchida, T. Okajima, K. Furukawa, T. Ando, H. Ishida, A. Yoshida, Y. Nakamura, R. Kannagi, M. Kiso, K. Furukawa, J. Biol. Chem. 2003, 278, 22787-22794.

Regulation of sialyl Lewis antigen expression in colon cancer cells by sialidase NEU4

K. Shiozaki, K. Yamaguchi, K. Takahashi, S. Moriya, T. Miyagi, J. Biol. Chem. 2011, 286, 21052-21061.

Tumor-associated carbohydrate antigens: Sialyl Lea and T/Tn antigens in canine mammary tumors

M. Nowak, J. Madej, P. Dziegiel, W. Lopuszynski, A. Rodo, M. Ugorski, Vet. Pathol. 2009, 46, 222-226.

Role of mucin Lewis status in resistance to Helicobacter pylori infection in pediatric patients

S. Linden, C. Semino-Mora, H. Liu, J. Rick, A. Dubois, Helicobacter 2010, 15, 251-258.

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