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CAS RN: | Product Number: R0228

Recombinant Sclerotium rolfsii lectin (= rSRL) expressed in Escherichia coli


Purity:
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1ML
€162.00
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Note:

[Handling Notes]
Avoid multiple freeze-thaw cycles. Add some preservative, e.g. sodium azide upon opening for the first time, and store at 2-10 °C.


Product Information
Product code: R0228
Product name: Recombinant Sclerotium rolfsii lectin (= rSRL) expressed in Escherichia coli
Quantity: 1 ml per vial (1 mg/1 ml: Lowry method)
Product form: Purified recombinant SRL, Liquid
Buffer: Phosphate buffered saline [PBS] (-), pH7.4
Molecular Mass: 16 kDa
Preservative : None
Stabilizer: None

Description
The rSRL is a carbohydrate-binding protein (Lectin) derived from Sclerotium rolfsii. The rSRL gene product is expressed in Escherichia coli. This lectin preferably binds to two types [GlcNAcβ(1-2)Man, Galβ(1-3)GalNAc] of glycan epitopes by its two distinct carbohydrate-binding sites. Consequently, rSRL exhibits a hemagglutination activity to a rabbit reticulocyte through the glycan binding to subsites and subunits of the lectin.

Storage
Store at -20°C to -80°C. Avoid repetitive freezing and thawing. After thawing the product, it's recommended to store at 2-10°C with an additional preservative (e.g., 0.02-0.05% [w/v] sodium azide).

Related Products
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R0225 Recombinant Polyporus squamosus lectin (=rPSL1a) expressed in Escherichia coli
R0226 Recombinant Laetiporus sulphureus lectin N-Terminal Domain (= rLSL-N) expressed in Escherichia coli
R0227 Recombinant Marasmius oreades agglutinin (= rMOA) expressed in Escherichia coli
R0229 Recombinant Griffithsia sp. lectin (= rGRFT) expressed in Escherichia coli
L0169 Lectin, Fucose specific (= AOL) from Aspergillus oryzae (5mg/mL, PBS pH6.5)

Product Number R0228
Physical State (20 deg.C) Liquid
Storage Temperature -20°C
Condition to Avoid Heat Sensitive
Specifications
Appearance Colorless to Light yellow to Light orange clear liquid
Concentration(Lowry Methoed) 0.9 to 1.5 mg/mL
Molecular weight 10000 to 25000(SDS-PAGE)
Hemagglutination activity max. 12.5 micro g/mL(pH7.2)
Properties (reference)
GHS
Related Laws:
Transport Information:
HS Number 3504009000
Application
Recombinant lectin

References


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