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CAS RN: | Product Number: R0231

rLSL-N-Biotin [for Galβ(1-4)GlcNAc, poly LacNAc]

  • rLSL-N-Biotin Conjugate
  • Recombinant Laetiporus sulphureus lectin N-Terminal Domain (= rLSL-N)-Biotin Conjugate
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Product Information
Product code: R0231
Product name: rLSL-N-Biotin [for Galβ(1-4)GlcNAc, poly LacNAc]
Quantity: 1 ml per vial (1 mg/1 ml: Lowry method)
Product form: Biotin-immobilized recombinant LSL-N, Liquid
Buffer: Phosphate buffered saline [PBS] (-), pH7.4
Molecular Mass: >17 kDa
Preservative : None
Stabilizer: None

The rLSL-N is an N-terminal domain of carbohydrate-binding protein (Lectin) derived from Laetiporus sulphureus. The rLSL-N gene product is expressed in Escherichia coli. This lectin preferably binds to [Galβ(1-4)GlcNAc, poly LacNAc] glycan epitopes.
Biotin is covalently linked to the protein surface. Resulting band shift caused by biotinylation is checked by SDS-PAGE. (Unbiotinylated band is mostly not observed.) The rLSL-N-Biotin shows significant interaction with asialo human transferrin (asialo hTF) possessing [asialo] glycan epitopes by lectin-ELISA analysis. According to the lectin-blotting analysis, the rLSL-N-Biotin specifically binds to β(1-4)Gal-terminated hTF but not to ungalactosylated (at the non-reducing terminal) glycoproteins such as hTF, agalacto hTF and bovine RNase B.

Store at -20°C to -80°C. Avoid repetitive freezing and thawing. After thawing the product, it’s recommended to store at 2-10°C with an additional preservative (e.g., 0.02-0.05% [w/v] sodium azide).

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A2659 AOL-Biotin Conjugate

Product Number R0231
Physical State (20 deg.C) Liquid
Storage Temperature -80°C
Condition to Avoid Heat Sensitive
Appearance Colorless to Almost colorless clear liquid
Concentration(Lowry Methoed) 0.9 to 1.5 mg/mL
Molecular weight 10 to 25 kDa
Suitability for use in ELISA tests min. 1.0 (10 micro g/mL, OD450)
Properties (reference)
Related Laws:
Transport Information:
HS Number 3504009000
Recombinant lectin


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