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CAS RN: | Product Number: R0233

rSRL-Biotin [for GlcNAcβ(1-2)Man, Galβ(1-3)GalNAc]


Purity:
Synonyms:
  • rSRL-Biotin Conjugate
  • Recombinant Sclerotium rolfsii lectin (= rSRL)-Biotin Conjugate
Documents:
1ML
€287.00
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Product Information
Product code: R0233
Product name: rSRL-Biotin [for GlcNAcβ(1-2)Man, Galβ(1-3)GalNAc]
Quantity: 1 ml per vial (1 mg/1 ml: Lowry method)
Product form: Biotin-immobilized recombinant SRL, Liquid
Buffer: Phosphate buffered saline [PBS] (-), pH7.4
Molecular Mass: >16 kDa
Preservative : None
Stabilizer: None

Description
The rSRL is a carbohydrate-binding protein (Lectin) derived from Sclerotium rolfsii. The rSRL gene product is expressed in Escherichia coli. This lectin preferably binds to two types [GlcNAcβ(1-2)Man, Galβ(1-3)GalNAc] of glycan epitopes by its two distinct carbohydrate-binding sites.
Biotin is covalently linked to the protein surface. Resulting band shift caused by biotinylation is checked by SDS-PAGE. (Unbiotinylated band is mostly not observed.) The rSRL-Biotin shows significant interaction with agalacto human transferrin (hTF) possessing [GlcNAcβ(1-2)Man] glycan epitopes by lectin-ELISA analysis. According to the lectin-blotting analysis, the rSRL-Biotin specifically binds to β(1-2)GlcNAc-terminated hTF but not to non-β(1-2)GlcNAc-terminated glycoproteins such as hTF, asialo hTF and bovine RNase B.

Storage
Store at -20°C to -80°C. Avoid repetitive freezing and thawing. After thawing the product, it’s recommended to store at 2-10°C with an additional preservative (e.g., 0.02-0.05% [w/v] sodium azide).

Related Products
R0228 Recombinant Sclerotium rolfsii lectin (= rSRL) expressed in Escherichia coli
R0238 rSRL-LecBeads [for GlcNAcβ(1-2)Man, Galβ(1-3)GalNAc]
R0230 rPSL1a-Biotin [for Sia α(2-6)Gal]
R0231 rLSL-N-Biotin [for Galβ(1-4)GlcNAc, poly LacNAc]
R0232 rMOA-Biotin [for Galα(1-3)Gal]
R0234 rGRFT-Biotin [for Manα(1-2)Man]
A2659 AOL-Biotin Conjugate

Product Number R0233
Physical State (20 deg.C) Liquid
Storage Temperature -80°C
Condition to Avoid Heat Sensitive
Specifications
Appearance Colorless to Almost colorless clear liquid
Concentration(Lowry Methoed) 0.9 to 1.5 mg/mL
Molecular weight 10 to 25 kDa
Suitability for use in ELISA tests min. 1.0 (10 micro g/mL, OD450)
Properties (reference)
GHS
Related Laws:
Transport Information:
HS Number 3504009000
Application
Recombinant lectin

References


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