Published TCIMAIL newest issue No.197
Maximum quantity allowed is 999
To understand basic biology and develop new treatments for diseases, it is important to identify biological interaction networks, known as interactomes. Proximity labelling, specifically photoproximity labeling, is one of the most effective methods for achieving this.1) By irradiating light, short-lived reactive active species probes are generated. These probes diffuse through the solution and covalently bind to nearby biomolecules, thus imparting a tag.2,3,4,5,6) For instance, it has been reported that antibodies covalently linked to photoreactive catalysts can label the microenvironment surrounding antibody-bound proteins through activation of probes. This technique does not necessitate the use of genetic modification technology.2)
The labelling radius of the probes used in proximity labeling affects the scale and resolution of the interactome being mapped. We offer commercial products with different labeling radii for proximity labelling.
A diazirine group, which has a singlet carbene as the reactive active species, is extremely reactive to water. Therefore, the labeling time is only a few minutes and the labeling radius is around 50 nm.6) Arylazide groups, in contrast, generate triplet nitrenes as reactive species and exhibit slow reactivity towards water. As a result, the labeling process takes approximately 10 minutes, and a labeling radius of 50-100 nm can be achieved.6,7,8,9)
Additionally, we offer difunctional photoreactive labeling agents that can be photo-crosslinked at the diazirine moiety and have an alkyne moiety as building blocks.
Products
Biotinylation Reagents
Building Blocks without Biotin Moiety
Related Products
References
- 1) In Vivo Proximity Labeling for the Detection of Protein–Protein and Protein–RNA Interactions
- 2) Microenvironment mapping via Dexter energy transfer on immune cells
- 3) Photoproximity Labeling of Sialylated Glycoproteins (GlycoMap) Reveals Sialylation-Dependent Regulation of Ion Transport
- 4) Tracking chromatin state changes using nanoscale photo-proximity labelling
- 5) Photoproximity Labeling from Single Catalyst Sites Allows Calibration and Increased Resolution for Carbene Labeling of Protein Partners In Vitro and on Cells
- 6) Targeted activation in localized protein environments via deep red photoredox catalysis
- 7) Radius measurement via super-resolution microscopy enables the development of a variable radii proximity labeling platform
- 8) Photoaffinity labeling in target- and binding-site identification
- 9) Photoactivatable Lipid Probes for Studying Biomembranes by Photoaffinity Labeling
- 10) Labeling Preferences of Diazirines with Protein Biomolecules