Tetracycline Hydrochloride

Reference

• Susceptibility of Actinobacillus actinomycetemcomitans to six antibiotics decreases as biofilm matures
  • N. Takahashi, K. Ishihara, T. Kato, K. Okuda, J. Antimicrob. Chemother. 2007, 59, 59.


Application
DNA aptamer research

Reference

• Single-stranded DNA aptamers specific for antibiotics tetracyclines
• J.H. Niazi, S.J. Lee, M.B. Gu, Bioorg. Med. Chem. 2008, 16, 7245


Application
Tetracycline: a Broad Spectrum Polyketide Antibiotic and a Selective Reagent Genetically Engineered Cells
Tetracycline is a polyketide antibiotic isolated from Streptomyces genus. Tetracycline has broad activity against Gram-negative bacteria and Gram-positive bacteria, with a few exceptions, such as Pseudomonas aeruginosa and Proteus species. Tetracycline inhibits protein synthesis in susceptible organisms by binding to the A-site on the 50S subunit of a bacterial ribosome. However, tetracycline-resistant bacterial strains appeared soon after its introduction. Tetracycline is used in molecular biology as a selective reagent most commonly to isolate bacteria coupled to a gene coding for tetracycline resistance, which is one of the most frequently used selection markers for obtaining transgenic cells. Recently, tetracycline is used for tetracycline-controlled transcriptional activation systems in cells.

References

• Tetracyclines. Antibiotic action, uptake, and resistance mechanisms (a review)
  • D. Schnappinger, W. Hillen, Arch. Microbiol. 1996, 165, 359.
• Crystal structures of complexes of the small ribosomal subunit with tetracycline, edeine and IF3
  • M. Pioletti, F. Schlünzen, J. Harms, R. Zarivach, M. Glühmann, H. Avila, A. Bashan, H. Bartels, T. Auerbach, C. Jacobi, T. Hartsch, A. Yonath, F. Franceschi, EMBO J. 2001, 20, 1829.
• The structural basis for the action of the antibiotics tetracycline, pactamycin, and hygromycin B on the 30S ribosomal subunit
  • D. E. Brodersen, W. M. Clemons, A. P. Carter, R. J. Morgan-Warren, B. T. Wimberly, V. Ramakrishnan, Cell 2000, 103, 1143.
• Tetracycline resistance determinants: mechanisms of action, regulation of expression, genetic mobility, and distribution (a review)
  • M. C. Roberts, FEMS Microbiol. Rev. 1996, 19, 1.
• Update on acquired tetracycline resistance genes
  • M. C. Roberts, FEMS Microbiol. Lett. 2005, 245, 195.
• In vivo gene regulation using tetracycline-regulatable systems
  • K. Stieger, B. Belbellaa, C. Le Guiner, P. Moullier, F. Rolling, Adv. Drug Delivery Rev. 2009, 61, 527.


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