Clearing Reagent iCBiofilm-H1 [for Biofilm]

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Synonymes

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SDS | Spécifications | CoA et autres Certificats | Graphiques analytiques

Informations générales | Spécifications et Propriétés | Sécurité et Réglementaire | Applications & Litterature

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Taille	Prix ​​unitaire	Belgique	Japon *	Quantité	Informations d'expédition
8ML	64,00 €	Contactez-nous	33		Simulation d'expédition

*Stock disponible en Belgique : Expédition le jour même
*Stock disponible au Japon : Veuillez consulter la simulation d'expédition pour une estimation des délais d'expédition (hors articles réglementés et expédition avec Carboglace)

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Product Information

• Size : 8 mL

Description

• Biofilms are highly organized structures of microbial communities composed of a matrix of polysaccharides and other substances secreted by microbes and/or aggregates of microbial cells. Microbial cells become resistant to antimicrobial drugs and host defense systems when they form biofilms. Clearing Reagent iCBiofilm-H1 has been developed to allow for the observation of biofilm structure at thicknesses of more than several tens of micrometers. Simple addition of iCBiofilm-H1 solution to a biofilm renders it transparent within several seconds, allowing for easy observation using light microscopy techniques.
  *This product was developed by Associate Prof. Sugimoto of the Jikei University School of Medicine.

Storage

• Store at room temperature. Use within 6 months.

Direction for Use

• 1. Prepare a biofilm in a glass bottomed culture dish (35mm diam.). Culture conditions (including medium, temperature, time, etc.) all play roles in biofilm formation; the conditions specific to your microbe of interest must be determined separately.
  2. After removing planktonic cells and culture medium, add 1% glutaraldehyde solution in PBS and fix at room temperature for 30 minutes. To preserve immunogenicity for immunofluorescence applications, we recommend substituting 4% paraformaldehyde in PBS.
  3. Remove these glutaraldehyde solution and wash three times with PBS or distilled water.
  4. Stain using appropriate fluorescence reagents.
  5. Remove staining reagents and add 500 µL – 1 mL of Clearing Reagent iCBiofilm-H1 (Product No.T4031).
  6. Observe biofilm using optical microscopy.

Related Products

• T4032 Clearing Reagent iCBiofilm-H2 [for Biofilm]
• I1275 Clearing Reagent iCBiofilm-D [for Biofilm]
• G0068 Glutaraldehyde (ca. 50% in Water, ca. 5.6mol/L)