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CAS RN: 59793-96-3 | Product Number: A1844

endo-α-N-Acetylgalactosaminidase (=Endo-α) Recombinant: from Bifidobacterium longum expressed in Escherichia coli


Purity:
Synonyms:
  • Endo-α Recombinant: from Bifidobacterium longum expressed in Escherichia coli
Documents:
1VIAL
£316.00
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Note:

Product Information

  • Quantity : 100 mU per vial
  • Product Form : Purified Endo-α, Lyophilized powder
  • Preservative : None
  • Stabilizer : None

Description

  • This Endo-α is a glycoside hydrolase that recognizes the structure of the Galβ1-3GalNAc disaccharide α-linked with a hydroxyl group and it releases Galβ1-3GalNAc (galacto-N-biose) by hydrolysis. The Endo-α gene is derived from Bifidobacterium longum, and its recombinant protein is expressed in Escherichia coli.

Unit Definition

  • One unit (U) of the Endo-α is defined as the amount required to produce 1 μmol of Galβ1-3GalNAc from Galβ1-3GalNAc α-pNP (Product No. G0375) per minute at 37°C under the condition of pH 5.0.

Assay Conditions

  • Reaction conditions: 50 mM Sodium acetate, pH 5.0
    The reaction mixture includes the enzyme and Galβ1-3GalNAc α-pNP in 20 μL. The mixture is incubated at 37°C for 10 minutes. After the enzyme reaction, quenching is performed by adding 30 μL of 0.25 M sodium carbonate. Quantification of released pNP is measured by a UV spectrometer.

Storage

  • Store at -80°C in its lyophilized powder form. Use promptly after dissolving in buffer.

Related Products

  • I1205 Sialidase (Neuraminidase)
  • I1206 Sialidase (Neuraminidase) Agarose
  • G0622 O-Glycosidase for core1 (Endo-α and Sialidase mix)

  • Other oligosaccharide-releasing enzymes
  • [Endo-type N-glycanase (ENGase)]
  • A1651 Endo-M (for hydrolysis of non core-fucosylated biantennary N-glycan)
  • E1339 Endo-M-W251N (for hydrolysis of core-fucosylated biantennary N-glycan)
  • G0365 Glycosynthase (for transglycosylation of non core-fucosylated biantennary N-glycan)

  • [Endo-type O-glycanase]
  • A1844 Endo-α (for hydrolysis of core1 O-glycan epitope known as T/TF antigen)

  • [Endo-type glycoceramidase]
  • R0240 rEGCase I
  • R0241 rEGCase I assisted by Activator II
  • R0242 rEGCase II
  • R0243 rEGCase II assisted by Activator II

  • [Endo-type glycosaminoglycanase]
  • K0069 Keratanase II (for hydrolysis of Keratan sulfate)

Product Number A1844
Physical State (20 deg.C) Solid
Storage Temperature Frozen (-80°C)
Condition to Avoid Heat Sensitive
CAS RN 59793-96-3
Enzyme Commission Number 3.2.1.97
MDL Number

MFCD00131003

Specifications
Appearance White powder to crystal
Titer 80 ~ 120 mUnit/1 Vial
Properties (reference)
GHS
Related Laws:
Transport Information:
HS Number 3507909090
Application
Argatroban: A Direct Thrombin Inhibitor Act as an Anticoagulant

References


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