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CAS RN: 504-02-9 | Product Number: A5581

1,3-Cyclohexanedione [for HPLC Labeling]


Purity: >98.0%(GC)(T)
Synonyms:
Documents:
5G
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Product Number A5581
Purity / Analysis Method >98.0%(GC)(T)
Molecular Formula / Molecular Weight C__6H__8O__2 = 112.13  
Physical State (20 deg.C) Solid
Storage Temperature 0-10°C
Store Under Inert Gas Store under inert gas
Condition to Avoid Light Sensitive,Air Sensitive,Heat Sensitive
CAS RN 504-02-9
Reaxys Registry Number 385888
PubChem Substance ID 87563197
SDBS (AIST Spectral DB) 10165
Merck Index (14) 3178
MDL Number

MFCD00001585

Specifications
Appearance White to Orange to Green powder to crystal
Purity(GC) min. 98.0 %
Purity(Neutralization titration) min. 98.0 %
Melting point 104.0 to 108.0 °C
Solubility in Water almost transparency
Effect test of HPLC derivatization Effective as labeling agent for Formaldehyde, Acetaldehyde, Propionaldehyde, n-Butyraldehyde, n-Valeraldehyde
Properties (reference)
Melting Point 106 °C
GHS
Pictogram Pictogram
Signal Word Warning
Hazard Statements H302 : Harmful if swallowed.
Precautionary Statements P501 : Dispose of contents/ container to an approved waste disposal plant.
P270 : Do not eat, drink or smoke when using this product.
P264 : Wash skin thoroughly after handling.
P301 + P312 + P330 : IF SWALLOWED: Call a POISON CENTER/doctor if you feel unwell. Rinse mouth.
Related Laws:
EC Number 207-980-0
RTECS# GV0350000
Transport Information:
HS Number 2914290090
Application
HPLC Labeling Reagent for Carbonyl Compounds

The compound 1 is an HPLC fluorescence labeling reagent, and can easily react with a carbonyl groups to form the corresponding decahydroacridine-1,8-dion (DHA) derivative. The resultant derivative is stable enough to reach the detector without any decomposition under reversed phase HPLC. An excellent chromatogram can be obtained by fluorescence detection analysis at the excitation and emission wavelengths of 366 nm and 440 nm, respectively.

Application example:
[Aliphatic aldehydes] 1,2)
5 mL of acetic acid and 10 g of ammonium acetate are dissolved in distilled water. Then 0.25 g of labeling reagent 1 is added to the solution and shaken to prepare the derivatization reagent solution. 2 mL of this solution is added to 1 mL of aqueous solution (ethanol solution, in the case of long-chain aldehydes) containing 10~30 ng of an aliphatic aldehyde, and incubate at 60 °C for 30 min. After cooling, use 1 µL of this solution as an HPLC sample.

References


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