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CAS RN: 56-75-7 | Product Number: C2255


Purity: >98.0%(T)(HPLC)
  • D-(-)-threo-2-Dichloroacetamido-1-(4-nitrophenyl)-1,3-propanediol
  • 2,2-Dichloro-N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)-2-propyl]acetamide
9   ≥40 
1   7  

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Product Number C2255
Purity / Analysis Method >98.0%(T)(HPLC)
Molecular Formula / Molecular Weight C__1__1H__1__2Cl__2N__2O__5 = 323.13 
Physical State (20 deg.C) Solid
Condition to Avoid Light Sensitive
CAS RN 56-75-7
Reaxys Registry Number 2225532
PubChem Substance ID 87560304
Merck Index (14) 2077
MDL Number


Appearance White to Orange to Green powder to crystal
Purity(HPLC) min. 98.0 area%
Purity(Argentometric Titration) min. 98.0 %
Melting point 149.0 to 153.0 °C
Specific rotation [a]20/D +17.0 to +21.0 deg(C=5, EtOH)
Properties (reference)
Melting Point 151 °C
Specific Rotation 20° (C=5,EtOH)
Solubility in water Practically insoluble
Solubility (very soluble in) Acetone,Methanol,Ethanol
Solubility (soluble in) Chloroform,Ether
Solubility (insoluble in) Benzene
Pictogram Pictogram
Signal Word Danger
Hazard Statements H361 : Suspected of damaging fertility or the unborn child.
H372 : Causes damage to organs through prolonged or repeated exposure.
H373 : May cause damage to organs through prolonged or repeated exposure.
H340 : May cause genetic defects.
H350 : May cause cancer.
Precautionary Statements P501 : Dispose of contents/ container to an approved waste disposal plant.
P260 : Do not breathe dust/ fume/ gas/ mist/ vapours/ spray.
P202 : Do not handle until all safety precautions have been read and understood.
P201 : Obtain special instructions before use.
P280 : Wear protective gloves/ protective clothing/ eye protection/ face protection.
P308 + P313 : IF exposed or concerned: Get medical advice/ attention.
Related Laws:
EC Number 200-287-4
RTECS# AB6825000
Transport Information:
HS Number 2941400000
Chloramphenicol: A Broad Spectrum Antibiotic and a Selective Reagent Genetically Engineered Cells

Chloramphenicol was originally isolated from the soil bacterium Streptomyces venezuelae in 1947. Chloramphenicol and a congener thiamphenicol [T2802] can now be made by chemical synthesis. Chloramphenicol has a highly stable which can be stored for prolonged times at room temperature. It has a broad spectrum of antibiotic activity including Staphylococcus aureus, Streptococcus pneumoniae, and Escherichia coli, while it is not effective against Pseudomonas aeruginosa. Chloramphenicol inhibits protein synthesis in susceptible organisms by binding to specific nucleotides in 23S rRNA within the 50S subunit of a bacterial ribosome. However, chloramphenicol causes side effects including bone marrow suppression and aplastic anemia. Chloramphenicol is used in molecular biology as a selective reagent most commonly to isolate bacteria coupled to a gene coding for chloramphenicol resistance, which is one of the most frequently used selection markers for obtaining transgenic cells.


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