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CAS RN: | Product Number: C3384

4-Chloro-1-naphthol (Ready-to-use solution) [for Western blotting]

  • 4-CN (Ready-to-use solution)
1   12  

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Product Information

Volume : 100 mL


This product is supplied as a ready-to-use solution containing 4-Chloro-1-naphthol (4-CN) and hydrogen peroxide for use in the visualization of membrane-bound horseradish peroxidase (HRP)-conjugated antibodies in western blotting. When this product reacts with HRP, a blue-purple precipitate is formed.

Directions for Use

1. : Allow HRP-conjugated antibody to bind membrane-bound protein of interest.

2. : Apply a thin layer of 4-CN solution (Product No. C3384) to the membrane via pipette and incubate until color appears.

3. : After bands appear, add deionized water to dilute 4-CN solution and stop color development.


Store at 4 °C. Do not freeze.

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Product Number C3384
Physical State (20 deg.C) Liquid
Storage Temperature 0-10°C
Condition to Avoid Heat Sensitive
Related CAS RN 604-44-4
Appearance Colorless to Almost colorless clear liquid
Peroxidase detection test to pass test
Properties (reference)
Pictogram Pictogram Pictogram
Signal Word Danger
Hazard Statements H302 + H332 : Harmful if swallowed or if inhaled.
H319 : Causes serious eye irritation.
H360 : May damage fertility or the unborn child.
H370 : Causes damage to organs.
H372 : Causes damage to organs through prolonged or repeated exposure.
Precautionary Statements P260 : Do not breathe dust/ fume/ gas/ mist/ vapours/ spray.
P201 : Obtain special instructions before use.
P264 : Wash skin thoroughly after handling.
P280 : Wear protective gloves/ protective clothing/ eye protection/ face protection.
P308 + P311 : IF exposed or concerned: Call a POISON CENTER/doctor.
P304 + P340 + P312 : IF INHALED: Remove person to fresh air and keep comfortable for breathing. Call a POISON CENTER/doctor if you feel unwell.
Related Laws:
EC Number 210-068-5
Transport Information:
HS Number 3822190090
4-Chloro-1-naphthol (Ready-to-use solution) [for Western blotting]


  • Cold Spring Harbor Laboratory Press, New York
    • E. Harlow and D. Lane., Antibodies: A Laboratory Manual, 1988,404,506,594
  • Humana Press, Totowa, New Jersey
    • J. M. Walker, The Protein Protocols Handbook, 2nd ed., 2002, 415-418

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