CAS RN: | Product Number: A3176
AzBTS (Ready-to-use solution) [for ELISA]
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Volume : 100 mL
This product is supplied as a ready-to-use solution containing 2,2'-Azinobis (3-ethylbenzothiazoline-6-sulfonic Acid Ammonium Salt) (AzBTS) and hydrogen peroxide (H2O2) for ELISA. When this product is reacted with horseradish peroxidase (HRP), a green soluble reaction product appears. Its absorbance can be measured at 405 nm.
Direction for Use
The following steps are for a 96-well plate format.
1. Bring AzBTS solution (Product No. A3176) to room temperature.
2. Gently mix the AzBTS solution (Product No. A3176).
3. Add 100uL of AzBTS solution (Product No. A3176) to each well.
4. Incubate the plate at room temperature for 30 minutes.
5. Within 1 hour from start the reaction, measure the absorbance of each well at 405 nm.
The product should be stored at 4 ºC, and used within 6 months. Don’t freeze because of arising precipitate.
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|Physical State (20 deg.C)||Liquid|
|Condition to Avoid||Heat Sensitive|
|Peroxidase detection test||Extent of coloring same as reference|
|Hazard Statements||H315 : Causes skin irritation.
H319 : Causes serious eye irritation.
|Precautionary Statements||P264 : Wash skin thoroughly after handling.
P280 : Wear protective gloves/ eye protection/ face protection.
P302 + P352 : IF ON SKIN: Wash with plenty of water.
P337 + P313 : If eye irritation persists: Get medical advice/ attention.
P305 + P351 + P338 : IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P362 + P364 : Take off contaminated clothing and wash it before reuse.
P332 + P313 : If skin irritation occurs: Get medical advice/ attention.
|Customs Control Conditions (Q)|
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