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CAS RN: 131467-87-3 | Product Number: A5557

NBD-H (=4-Hydrazino-7-nitro-2,1,3-benzoxadiazole Hydrazine) [for HPLC Labeling]


Purity: >98.0%(HPLC)
Synonyms:
  • 4-Hydrazino-7-nitrobenzofurazan Hydrazine
  • 4-Hydrazino-7-nitro-2,1,3-benzoxadiazole Hydrazine
Documents:
100MG
€125.00
4   14  

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Product Number A5557
Purity / Analysis Method >98.0%(HPLC)
Molecular Formula / Molecular Weight C__6H__5N__5O__3·H__4N__2 = 227.18  
Physical State (20 deg.C) Solid
Storage Temperature 0-10°C
Store Under Inert Gas Store under inert gas
Condition to Avoid Light Sensitive,Air Sensitive,Heat Sensitive
CAS RN 131467-87-3
PubChem Substance ID 87563175
MDL Number

MFCD00191508

Specifications
Appearance Orange to Brown powder to crystal
Purity(HPLC) min. 98.0 area%
Effect test of HPLC derivatization Effective as labeling agent for Propionaldehyde
Properties (reference)
GHS
Pictogram Pictogram
Signal Word Warning
Hazard Statements H315 : Causes skin irritation.
H319 : Causes serious eye irritation.
Precautionary Statements P264 : Wash skin thoroughly after handling.
P280 : Wear protective gloves/ eye protection/ face protection.
P302 + P352 : IF ON SKIN: Wash with plenty of water.
P337 + P313 : If eye irritation persists: Get medical advice/ attention.
P362 + P364 : Take off contaminated clothing and wash it before reuse.
P332 + P313 : If skin irritation occurs: Get medical advice/ attention.
Related Laws: / Compliance with laws, Regulations
Transport Information:
HS Number 2934999090
Application
HPLC Labeling Reagent for Carbonyl Compounds

The compound 1 is an HPLC fluorescence labeling reagent, which has a 2,1,3-benzoxadiazole skeleton and a hydrazino group, easily reacts with a carbonyl group to form the corresponding hydrazone. The labeling reagent itself is non-fluorescent, but the hydrazones after the reaction with carbonyl compounds have strong fluorescence. The resultant hydrazone is stable enough to reach the detector without any decomposition under reversed phase HPLC. An excellent chromatogram can be obtained by fluorescence detection at the excitation and emission wavelengths of 470 nm and 550 nm, respectively. Since their excitation and fluorescence wavelengths are at longer wavelengths, detection has less interference by contaminants, and a highly sensitive detection can be done because of its high reactivity.

Application examples:
[Aldehydes or ketones] 1)
250 µM of the labeling reagent 1 and 1.7 µM propionaldehyde are added to acetonitrile containing 0.025% TFA, and reacted at room temperature for 1 h, use it as the HPLC sample.
For example, the detection limit for propionaldehyde is 35 fmol.

References


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