CAS RN: 147611-81-2 | Product Number: A5575
DBD-NCS [=4-(N,N-Dimethylaminosulfonyl)-7-isothiocyanato-2,1,3-benzoxadiazole] [for HPLC Labeling and Edman Degradation]
*Stock available in Belgium will be delivered in 1 to 3 days
*Stock available in Japan will be delivered in 1 to 2 weeks (excludes regulated items and dry ice shipments).
|Appearance||Light orange to Yellow to Green powder to crystal|
|Elemental analysis(Nitrogen)||19.1 to 20.0 %|
|Melting point||124.0 to 128.0 °C|
|Effect test of HPLC derivatization||Effective as labeling agent for L-Phenylalanine|
|Melting Point||126 °C|
|Hazard Statements||H315 : Causes skin irritation.
H319 : Causes serious eye irritation.
|Precautionary Statements||P264 : Wash skin thoroughly after handling.
P280 : Wear protective gloves/ eye protection/ face protection.
P302 + P352 : IF ON SKIN: Wash with plenty of water.
P337 + P313 : If eye irritation persists: Get medical advice/ attention.
P362 + P364 : Take off contaminated clothing and wash it before reuse.
P332 + P313 : If skin irritation occurs: Get medical advice/ attention.
[Method by Manual Edman Degradation]
Peptide (insulin Chain B 500 pmol)
│ ・ Dissolve in 20 µL of 50% pyridine / H2O.
│ ・ Add 5 µL of 1% triethylamine / CH3CN and 10 µL of 20 mM HPLC labeling reagent 1 / pyridine,
│ and react the mixture at 50 °C for 15 min under the atmosphere of inert gas.
│ ・ After cooling to room temperature, wash the reactant solution 3 times
│ with 200 µL of heptane / dichlorormethane (6/4).
│ ・ Dry the washed solution at 50 °C for 15 min by using a centrifugation evaporator.
│ ・ Add 30 µL of 1% BF3·Et2O / CH3CN to the mixture and incubate the mixture at 50 °C for 5 min.
│ ・ Further dry the reactant solution under nitrogen gas.
│ ・ Add 20 µL of H2O, and then extract 2 times with 100 µL of benzene / AcOEt (1/4).
│―――――(Aqueous phase) A peptide will be eluted out.
│ ・ Dry the extracted organic phase under nitrogen gas.
│ ・ Dissolve the mixture in 2 µL of CH3CN.
│ ・ Add 8 µL of 0.4 M HCl and hydrolyze the mixture at 50 °C for 5 min.
│ ・ Treat the reactant with 5 µL of 4 M HCl and 0.5 M NaNO2 at room temperature for 10 min
│ and oxidize it.
│ ・ Neutralize the reactant with 23 µL of 1 M NaNO2, and remove an excessive oxidant
│ by adding 20 µL of 0.15 M methionine.
Use 20 µL of this solution as an HPLC sample solution.
HPLC Labeling Reagents ( PDF 10MB )
- 1)Y. Huang, H. Matsunaga, A. Toriba, T. Santa, T. Fukushima, K. Imai, Anal. Biochem. 1999, 270, 257.
- 2)H. Matsunaga, T. Santa, K. Hagiwara, H. Homma, K. Imai, S. Uzu, K. Nakashima, S. Akiyama, Anal. Chem. 1995, 67, 4276.
- 3)K. Imai, S. Uzu, K. Nakashima, S. Akiyama, Biomed. Chromatogr. 1993, 7, 56.
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