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CAS RN: 254973-02-9 | Product Number: A5576

AABD-SH (=4-Acetamido-7-mercapto-2,1,3-benzoxadiazole) [for HPLC Labeling]

Purity: >95.0%(HPLC)
  • 4-Acetamido-7-mercaptobenzofurazan
  • 4-Acetamido-7-mercapto-2,1,3-benzoxadiazole
1   ≥20 

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Product Number A5576
Purity / Analysis Method >95.0%(HPLC)
Molecular Formula / Molecular Weight C__8H__7N__3O__2S = 209.22  
Physical State (20 deg.C) Solid
Storage Temperature 0-10°C
Store Under Inert Gas Store under inert gas
Condition to Avoid Air Sensitive,Heat Sensitive
CAS RN 254973-02-9
PubChem Substance ID 87563193
MDL Number


Appearance Light yellow to Brown powder to crystal
Purity(HPLC) min. 95.0 area%
Effect test of HPLC derivatization Effective as labeling agent for Caproic Acid, Lauric Acid, Myristic Acid, Palmitic Acid
Properties (reference)
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Transport Information:
HS Number 2934999090
HPLC Labeling Reagent for Carboxylic Acids

The compound 1, an HPLC fluorescence labeling reagent, which has a 2,1,3-benzoxadiazole skeleton and a mercapto group, easily reacts with a carboxyl group to form the corresponding thioester. 1 itself fluoresces very little, but the thioester derivatives fluoresce highly. The resultant thioester is stable enough to reach the detector without any decomposition under reversed phase HPLC. An excellent chromatogram can be obtained by fluorescence detection at the excitation and emission wavelengths of 368 nm and 524 nm, respectively. [The detection limit = 10~20 fmol (S/N = 3)]

Application example:
Add 20 µL of mixed fatty acid / acetonitrile solution, 20 µL of 20 mM labeling reagent 1 / dichloromethane solution, 20 µL of triphenylphosphine / acetonitrile solution and 20 µL of 2,2’-dipyridyl disulfide / acetonitrile solution to a 500 µL vessel, and the mixture is left at room temperature for 15 min. Dilute this reactant mixture with 20 µL of acetonitrile, and use 1 µL of this diluted solution as an HPLC sample solution.


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