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CAS RN: 10199-89-0 | Product Number: A5592

NBD-Cl (=4-Chloro-7-nitro-2,1,3-benzoxadiazole) [for HPLC Labeling]

Purity: >98.0%(T)
  • 4-Chloro-7-nitrobenzofurazan
  • 4-Chloro-7-nitro-2,1,3-benzoxadiazole
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Product Number A5592
Purity / Analysis Method >98.0%(T)
Molecular Formula / Molecular Weight C__6H__2ClN__3O__3 = 199.55  
Physical State (20 deg.C) Solid
CAS RN 10199-89-0
Reaxys Registry Number 614212
PubChem Substance ID 87563199
SDBS (AIST Spectral DB) 5444
MDL Number


Appearance Light orange to Yellow to Green powder to crystal
Purity(Butylamine Method) min. 98.0 %
Melting point 97.0 to 100.0 °C
Solubility in Methanol almost transparency
Effect test of HPLC derivatization Effective as labeling agent for Butylamine , Propylamine
Properties (reference)
Melting Point 98 °C
Pictogram Pictogram
Signal Word Warning
Hazard Statements H315 : Causes skin irritation.
H319 : Causes serious eye irritation.
Precautionary Statements P264 : Wash skin thoroughly after handling.
P280 : Wear protective gloves/ eye protection/ face protection.
P302 + P352 : IF ON SKIN: Wash with plenty of water.
P337 + P313 : If eye irritation persists: Get medical advice/ attention.
P362 + P364 : Take off contaminated clothing and wash it before reuse.
P332 + P313 : If skin irritation occurs: Get medical advice/ attention.
Related Laws: / Compliance with laws, Regulations
Enzyme Commission 233-496-4
RTECS# DF8002400
Transport Information:
HS Number 2934999090
HPLC Labeling Reagent for Amines and Thiols

The compound 1 which is an HPLC fluorescence labeling reagent having a 2,1,3-benzoxadiazole skeleton, can easily react with a secondary amine and thiol. The resultant derivative is stable enough to reach the detector without any decomposition under general reversed phase HPLC. An excellent chromatogram can be obtained by fluorescence detection analysis at the excitation and emission wavelengths of 460 nm and 535 nm, respectively.

Application example:
[Alkylamines] 1)
To 25~500 µL of a methanol solution containing an amine (1~20 µg), 4~8 eq. excess amount of 0.05% labeling reagent 1 / methanol solution is added. After adding 50~100 µL of 0.1 M NaHCO3, incubate at 55 °C for 1~5 h. After cooling the reaction mixture to room temperature, use it as an HPLC sample.
TLC and HPLC of N-methylcarbamates, N,N-dimethylcarbamates in agrichemicals2,3) Hydrolyze the carbamates to label the amine derivatives.
TLC of amphetamines in urine4,5), HPLC of prolines (precolumn derivatization method)6)


PubMed Literature

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