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Published TCIMAIL newest issue No.198
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CAS RN: | Product Number: T4436
T-Fluor 480 Solution [for RNA staining]
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Synonyms:
Product Documents:
| Size | Unit Price | Belgium | Japan* | Quantity |
|---|---|---|---|---|
| 5ML |
€80.00
|
Contact Us | Contact Us |
|
*Stock available in Belgium will be delivered in 1 to 3 days
*Stock available in Japan will be delivered in 1 to 2 weeks (excludes regulated items and dry ice shipments).
Supplemental Product Information:
Product Information
- Size : 5 mL
- Sterility : Filter-sterilized
Description
- Stains RNA in bacteria.
- T4436 contains T-Fluor 480 dissolved in PBS at a concentration of 800 µg/mL.
- Wavelengths of RNA stained with T-Fluor 480: λex,max = 450 nm, λem,max = 485 nm.
- Very high fluorescence intensity. The signal can be observed without washing.
- Tested for RNA staining.
Directions for Use: RNA staining of E. coli with T-Fluor 480
- Cultivate E. coli BW25113 on LB medium at 37 °C overnight.
- Add 1/100 volume of culture medium to LB medium with or without 100 µg/mL rifampicin (RNA synthesis inhibitor) and incubate at 37 °C for 4 hours.
- Dilute T4436 125-fold with PBS.
- After removing the supernatant by centrifugation, add the 125-fold diluted T-Fluor 480 solution to the pellet and stain at room temperature for 5 minutes.
- Observe under a fluorescence microscope using a filter for GFP.
Directions for Use: Observation of RNA in transparent biofilm
- Incubate Methicillin-resistant Staphylococcus aureus (MRSA) in a glass-bottomed dish for 24 hours to form a biofilm.
- Remove the culture medium and suspended bacteria. Then fix and wash the biofilm.
- Dilute T4436 100-fold with PBS.
- Add the 100-fold diluted T-Fluor 480 solution to the dish and stain the biofilm at room temperature for 30 minutes.
- Add PBS or biofilm clearing reagent iCBiofilm-H1 (Product No. T4031) to the dish and observed the biofilm.
Storage
- This product is delivered frozen but can be stored at 4 °C. Please use it within six months after refrigeration.
Related Products
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- T4032 Clearing Reagent iCBiofilm-H2 [for Biofilm]
- I1275 Clearing Reagent iCBiofilm-B [for Biofilm]
- T4072 T-Fluor 480 [for RNA staining]
- R0079 Rifampicin
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| Product Number | T4436 |
| Physical State (20 deg.C) | Liquid |
Storage Temperature 
|
Frozen (<0°C) |
| Condition to Avoid | Heat Sensitive |
Specifications
| Appearance | Colorless to Yellow clear liquid |
| Lambda max. | 410.0 to 414.0 nm(PBS) |
| Absorbance(E1%1cm) | min. 60.0 |
| RNA staining test | to pass test |
Properties (reference)
GHS
| Pictogram |
|
| Hazard Statements | H411 : Toxic to aquatic life with long lasting effects. |
| Precautionary Statements | P501 : Dispose of contents/ container to an approved waste disposal plant. P273 : Avoid release to the environment. P391 : Collect spillage. |
Related Laws:
Transport Information:
| UN Number | UN3082 |
| Class | 9 |
| Packing Group | III |
Product Documents (Note: Some products will not have analytical charts available.)
Safety Data Sheet (SDS)
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