9-Chloromethylanthracene [for HPLC Labeling]

The compound 1, an HPLC labeling reagent which has a chloromethyl group, easily reacts with a carboxyl group to form the corresponding ester in the presence of a base. The resultant ester is stable and can reach the detector without any decomposition under reversed phase HPLC. An excellent chromatogram can be obtained by measuring at 254 nm, an absorption wavelength that is generally used for UV detection. Furthermore, it has a characteristic fluorescence based on an anthracene skeleton, thus carboxylic acids can be detected with the detection limit of 2 fmol by fluorescence detection analysis at the excitation and emission wavelengths of 365 nm and 412 nm, respectively.

Application examples:
[Fatty acids]¹⁾
Dissolve 60 µg of a sample in 1 mL of DMF, and add 1 mL of tetramethylammonium hydroxide / DMF solution (1 x 10^-3 M) and 1 mL of the labeling reagent 1 / cyclohexane solution (5 x 10^-3 M). Close the cap of the reaction vessel and incubate the solution at 75 °C for 30 min. Cool the resultant solution to room temperature and use it as an HPLC sample.
The detection limit = 0.1 pmol (UV detection: 254 nm)
The detection limit = 2 fmol (Fluorescence detection: λex 365 nm, λem 412 nm)

TCI Products Pamphlet
HPLC Labeling Reagents ( PDF 10MB )