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CAS RN: | Product Number: B6105
6X Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]
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Purity:
Synonyms:
Product Documents:
| Size | Unit Price | Belgium | Japan* |
Shipping Information
|
|---|---|---|---|---|
| 10ML |
€38.00
|
2 | ≥100 |
* Stock available in Belgium: Shipment on the same day
* Stock available in Japan: Please check the Shipping Simulation for estimated shipments. (excludes regulated items and dry ice shipments)
Supplemental Product Information:
Product Information
- Volume : 10 mL
Description
- This product contains 12% LDS, 50% glycerol, 0.03% bromophenol blue and 0.375 M Tris HCl, pH approx. 6.8. Does not contain 2-Mercaptoethanol. 6X Sample Buffer is specially formulated for protein sample preparation based on the Laemmli Method of SDS-PAGE system. LDS is anionic detergent that may be used in place of SDS for electrophoresis, it is more soluble than SDS.
- Note: Dissolve the precipitation at 25-30 °C if precipitation occurs during storage.
Direction for Use
-
Preparation of non-reducing sample
- Mix a protein sample with the 6X sample buffer (5:1). If necessary, heat the mix solution and then cool down the tube at room temperature. Use the prepared solution for SDS-PAGE.
-
Preparation of reducing sample (reducing with 2-Mercaptoethanol)
- Add 30 μL of 2-Mercaptoethanol per 70 μL of 6X sample buffer. Mix thoroughly.
- Then mix a protein sample with the sample buffer (5:1) and heat to 95-100 °C for 5 minutes. Cool down the tube at room temperature. Use the prepared solution for SDS-PAGE.
Note: Reducing reagents: 2-Mercaptoethanol [for Electrophoresis] (Product No. M1948), DL-Dithiothreitol [for Electrophoresis] (Product No. D3647), etc.
Storage
- Store at room temperature.
Related Products
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- B3193 Coomassie Brilliant Blue G-250 [for Electrophoresis]
- B3194 Coomassie Brilliant Blue R-250 [for Electrophoresis]
- B5834 2X SDS-PAGE Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]
- B6104 4X SDS-PAGE Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]
- B6110 2X SDS-PAGE Sample Buffer Phenol Red (2-Mercaptoethanol free) [for Electrophoresis]
- C3488 Coomassie Brilliant Blue G-250 (Ready-to-use solution) [for Electrophoresis]
- D3647 DL-Dithiothreitol [for Electrophoresis]
- M1948 2-Mercaptoethanol [for Electrophoresis]
| Product Number | B6105 |
| Physical State (20 deg.C) | Liquid |
Storage Temperature 
|
Room Temperature (Recommended in a cool and dark place, <15°C) |
Specifications
| Appearance | Green to Dark green to Dark blue clear liquid to cloudy liquid |
| Functionality test | to pass test(Electrophoresis) |
Properties (reference)
| Specific Gravity (20/20) | 1.16 |
GHS
| Pictogram |
|
| Signal Word | Warning |
| Hazard Statements | H315 : Causes skin irritation. |
| Precautionary Statements | P264 : Wash skin thoroughly after handling. P280 : Wear protective gloves. P302 + P352 : IF ON SKIN: Wash with plenty of water. P362 + P364 : Take off contaminated clothing and wash it before reuse. P332 + P313 : If skin irritation occurs: Get medical advice/ attention. |
Related Laws:
Transport Information:
| HS Number | 3822190090 |
Application
6X Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]
References
- Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
- Dodecyl sulfate/polyacrylamide-gel electrophoresis at low pH values and low temperatures.
Documents
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Other Documents
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| CAS RN | 60-24-2 |
| Purity / Analysis Method: | >99.0%(GC) |
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