Maximum quantity allowed is 999
| Size | Unit Price | Belgium | Japan* |
|---|---|---|---|
| Request a Quote | Request a Quote |
* Stock available in Belgium: Shipment on the same day
* Stock available in Japan: Please check the Shipping Simulation for estimated shipments. (excludes regulated items and dry ice shipments)
This kit contains buffers and anti-NeuN antibody (positive control) for 3D nuclear staining of CUBIC-cleared tissues.
The difference between CUBIC-HV™1 3D immunostaining kit (Product No. C3708) and CUBIC-HV™1 3D immunostaining kit (Casein separately) (Product No. C3717):
CUBIC-HV™1 3D immunostaining kit (Product No. C3708) includes a buffer with casein, which can be used as soon as it is arrived. The buffer with casein should be used as soon as possible.
CUBIC-HV™1 3D immunostaining kit (Casein separately) (Product No. C3717) includes a buffer without casein and casein is in the kit separately. The casein should be mixed to the buffer just before use and after mixing, the buffer should be used as soon as possible. It takes about 2 hours to solve the casein.
Please see the detail protocols in CUBICStars’ web site.
https://www.cubicstars.com/cubic-hv/index.html
Constituents in kit (suitable for a brain of an adult mouse)
Process outline
====Delipidation====
Perfusion fixation and dissection of the mouse brain
Post-fix <24 h
Wash (PBS) 2 h x3
Delipidation (CUBIC-L; Product No. T3740) 3-7 days
Wash (PBS) 2 h x3
====Nuclear staining by CUBIC-HV™1 3D nuclear staining kit (Product No. C3709) ====
Nuclear staining (DAPI or Propidium Iodide or others in CUBIC-HV™1 3D nuclear staining buffer) 3-5 days
Wash (3D nuclear staining wash buffer; 10mM HEPES buffer, pH 7.5) 2 h x3
==== Optional process; Enzyme reaction ====
====Immunostaining by CUBIC-HV™1 3D immunostaining kit (Casein separately) (Product No. C3717) ====
Add the entire amount of casein powder in the kit (1 vial) to the 3D immunostaining buffer (without Casein). It takes about 2 hours at room temperature.
From this time on, 3D immunostaining buffer including Casein should be used.
Primary antibody (anti NeuN antibody in C3717 kit can be used): 5 µg/whole brain
Secondary antibody: anti-Fc Fab (out of the kits), 3.75 µg/whole brain
Primary antibody + secondary antibody reaction / Replacement with CUBIC-HV™1 3D immunostaining buffer, 1.5 h
Immunostaining (CUBIC-HV™1 3D immunostaining buffer with CUBIC-HV™1 3D immunostaining additive (if required)), >1 week
Reaction in the same buffer at 4°C, 1 day
Wash (CUBIC-HV™1 3D immunostaining wash buffer), 2 h x3
====Fix and RI matching====
Post-fix (in 1% Formaldehyde Solution), 1 day
Wash (PBS), 2 h
RI matching (CUBIC-R+(M); Product No. T3740), <3 days
Microscopic observation
Related Products
T3740 Tissue-Clearing Reagent CUBIC-L [for delipidation and decoloring]| Product Number | C3717 |
Storage Temperature 
|
Refrigerated (0-10°C) |
| Condition to Avoid | Heat Sensitive |
| Pictogram |
|
| Signal Word | Warning |
| Hazard Statements | H319 : Causes serious eye irritation. |
| Precautionary Statements | P264 : Wash skin thoroughly after handling. P280 : Wear eye protection/ face protection. P337 + P313 : If eye irritation persists: Get medical advice/ attention. P305 + P351 + P338 : IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. |
| HS Number | 3822190090 |
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