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CAS RN: 6850-28-8 | Product Number: T3294
Tris(hydroxymethyl)aminomethane Acetate
Purity: >99.0%(T)
Synonyms:
- 2-Amino-2-(hydroxymethyl)-1,3-propanediol Acetate
- Trimethylolaminomethane Acetate
- Tris Acetate
Product Documents:
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| Product Number | T3294 |
Purity / Analysis Method
|
>99.0%(T) |
| Molecular Formula / Molecular Weight | C__4H__1__1NO__3·C__2H__4O__2 = 181.19 |
| Physical State (20 deg.C) | Solid |
Storage Temperature
|
Room Temperature (Recommended in a cool and dark place, <15°C) |
| CAS RN | 6850-28-8 |
| Reaxys Registry Number | 3702918 |
| PubChem Substance ID | 253661155 |
| MDL Number | MFCD00038951 |
Specifications
| Appearance | White to Almost white powder to crystal |
| Purity(Nonaqueous Titration) | min. 99.0 % |
| Purity(Neutralization titration) | min. 99.0 % |
| Melting point | 118.0 to 122.0 °C |
| Absorbance | max. 0.500(290 nm, 50%H2O) |
Properties (reference)
| Melting Point | 120 °C |
| Solubility in water | Soluble |
GHS
Related Laws:
| EC Number | 229-939-6 |
Transport Information:
| HS Number | 2922190090 |
Application
Tris Acetate: A Reagent for the Preparation of TAE (Tris Acetate - EDTA) Buffer
Tris(hydroxymethyl)aminomethane acetate (tris acetate) is widely used as a component of buffer solutions, especially for solutions of nucleic acids. Tris acetate is used for the preparation of TAE (Tris acetate - EDTA) buffer, which is used as a running buffer and in agarose gels. TAE buffer is often used for DNA and RNA agarose gel electrophoresis.
References
- Choice of buffer anion for the assay of adenosine 5'-triphosphate using firefly luciferase
- Direct and Indirect Electroosmotic Flow Velocity Measurements in Microchannels
- Denaturing RNA electrophoresis in TAE agarose gels
PubMed Literature
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