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CAS RN: | Product Number: T3854
TMB [for ELISA] (Ready-to-use solution)
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Purity:
Synonyms:
- 3,3',5,5'-Tetramethylbenzidine [Soluble chromogenic substrate for ELISA] (Ready-to-use solution)
Product Documents:
| Size | Unit Price | Belgium | Japan* |
Shipping Information
|
|---|---|---|---|---|
| 100ML |
59,00 €
|
Contact Us | Contact Us |
* Stock available in Belgium: Shipment on the same day
* Stock available in Japan: Please check the Shipping Simulation for estimated shipments. (excludes regulated items and dry ice shipments)
Supplemental Product Information:
Product Information
Volume : 100 mL
Description
This product is supplied as a ready-to-use solution containing 3,3',5,5'-Tetramethyl -benzidine (TMB) and hydrogen peroxide for ELISA. When this product reacts with horseradish peroxidase (HRP), a blue colored soluble reaction product appears. Its absorbance can be measured at 650 nm. After terminating the reaction by adding hydrochloric acid, the reaction product becomes yellow and its absorbance can be measured at 450 nm.
Direction for Use
The following steps are for a 96-well plate format.
1. Bring TMB solution (Product No. T3854) to room temperature.
2. Gently mix the TMB solution (Product No. T3854).
3. Add 100uL of TMB solution (Product No. T3854) to each well.
4. Incubate the plate at room temperature for 30 minutes.
5. To terminate the reaction, add 100uL of 1 N HCl (Product No. H1202) to each well.
6. Within 1 hour of terminating the reaction, measure the absorbance of each well at 450 nm.
Storage
The product should be stored at 4 ºC, and used within 6 months.
Precipitations sometimes arise, but they do not affect the quality.
Related Products
T3855 TMB (Ready-to-use solution) [for Western blotting]
T2573 3,3',5,5'-Tetramethylbenzidine [for Biochemical Research]
H1202 Hydrochloric Acid (1mol/L)
H1621 Horseradish Peroxidase Maleimide Conjugate
A3075 Anti-6xHis Monoclonal Antibody (6A12) HRP Conjugate
A3187 Anti-Protein A Chicken Polyclonal Antibody HRP Conjugate
A3195 Anti-α Gal Chicken Polyclonal Antibody HRP Conjugate
G0407 Goat Anti-Mouse IgG HRP Conjugate
G0417 Goat Anti-Mouse IgM HRP Conjugate
G0418 Goat Anti-Rabbit IgG HRP Conjugate
P2466 Protein A HRP Conjugate
S0972 Streptavidin HRP Conjugate
S0999 Sheep Anti-Chicken IgY HRP Conjugate
| Product Number | T3854 |
| Physical State (20 deg.C) | Liquid |
Storage Temperature 
|
Refrigerated (0-10°C) |
| Condition to Avoid | Heat Sensitive |
Specifications
| Appearance | Colorless to Almost colorlessclear liquid |
| Peroxidase detection test | to pass test |
Properties (reference)
GHS
| Pictogram |
|
| Signal Word | Danger |
| Hazard Statements | H315 : Causes skin irritation. H319 : Causes serious eye irritation. H372 : Causes damage to organs through prolonged or repeated exposure. H370 : Causes damage to organs. |
| Precautionary Statements | P501 : Dispose of contents/ container to an approved waste disposal plant. P260 : Do not breathe mist or vapours. P264 : Wash skin thoroughly after handling. P280 : Wear protective gloves/ eye protection/ face protection. P337 + P313 : If eye irritation persists: Get medical advice/ attention. P308 + P311 : IF exposed or concerned: Call a POISON CENTER/doctor. |
Related Laws:
Transport Information:
| HS Number | 3822190090 |
Application
TMB [for ELISA] (Ready-to-use solution)
References
- Cold Spring Harbor Laboratory Press, New York
- E. Harlow and D. Lane., Antibodies, A Laboratory Manual, 1988, p404, p506, p594
Product Documents (Note: Some products will not have analytical charts available.)
Safety Data Sheet (SDS)
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Specifications
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Other Documents
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