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CAS RN: 89889-52-1 | Product Number: B6096

Biotin-LC-LC-NHS (2mg×5)

  • N-Succinimidyl N-[6-(Biotinamido)hexanoyl]-6-aminohexanoate (2mg×5)
  • N-[6-(Biotinamido)hexanoyl]-6-aminohexanoic Acid N-Succinimidyl Ester (2mg×5)
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Product Information
Volume/Quantity : 2 mg x 5
Molecular weight : 567.7

N-Hydroxysuccinimide (NHS) esters of biotin are commonly used as biotinylation reagents. B6096, Biotin-LC-LC-NHS reacts with the primary amines (-NH2) in buffers with pH between 7 and 9. The product is supplied in quantities suitable for biotinylation of up to 30 mg of antibodies.

This product should be stored at -20 ºC. Do not store at stock solution.

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Directions for Use
Use of a 10 mM Biotin-LC-LC-NHS solution is recommended. In order to efficiently biotinylate a sample, Biotin-LC-LC-NHS should be used at a 15-fold molar excess over the amount of amine-containing protein. Make sure to calculate the 10 mM biotin solution amount to be used with respect to the molar value of the sample (see example below).
* Do not store reagent in solution.

Calculate A uL of 10 mM biotinylation reagent solution :
For 2mg IgG (150,000 M.W.)
2 [mg IgG] x 10-3 [g/mg] x 1/150,000 [mol/g] x 15 [fold]
= A [uL of 10 mM B6096 solution] x 10-6 [L/uL] x 10 [mmol/L] x 10-3 [mol/mmol]

A = 20 [uL of 10 mM B6096 solution]

1. Bring Biotin-LC-LC-NHS (Product No. B6096) to room temperature.
2. Dissolve 2 mg of Biotin-LC-LC-NHS (Product No. B6096) in 350uL of dimethyl sulfoxide (DMSO) or dimethylformamide (DMF) and prepare a 10 mM solution of Biotin-LC-LC-NHS.
3. Dissolve the sample (1-10 mg/mL) in an amine-free buffer with pH between 7 and 9 such as PBS. Do not use buffers containing amines such as Tris.
4. Add A uL of 10 mM Biotin-LC-LC-NHS solution to the sample solution.
5. Incubate the mixed solution for 30 min at room temperature.
6. Remove unreacted and hydrolyzed reagent using desalting column or dialysis methods according to the manufacturer’s instructions.

Product Number B6096
Molecular Formula / Molecular Weight C__2__6H__4__1N__5O__7S = 567.7  
Physical State (20 deg.C) Solid
Storage Temperature -20°C
Store Under Inert Gas Store under inert gas
Condition to Avoid Moisture Sensitive,Heat Sensitive
CAS RN 89889-52-1
Reaxys Registry Number 8377428
MDL Number


Appearance White to Almost white powder to crystal
Functionality test to pass test
Properties (reference)
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