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CAS RN: | Product Number: C3698

CUBIC-HV™1 3D nuclear staining kit


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Note:

This product is discontinued. Please use a successor product, CUBIC-HV™1 3D nuclear staining kit (Product Number: C3709).

Please see the detail protocols in CUBICStars’ web site.
https://www.cubicstars.com/cubic-hv/index.html


Constituents in kit (suitable for a brain of an adult mouse)

  • CUBIC-HV™1 3D nuclear staining buffer (1x) (for 10 tests)
  • DAPI·2HCl (200x) (for 10 tests)
  • Propidium Iodide (100x) (for 10 tests)
  • 10 x 5 mL tube

Process outline

====Delipidation====

Perfusion fixation and dissection of the mouse brain
Post-fix ~24 h
Wash (PBS) 2 h x3
Delipidation (CUBIC-L; Product No. T3740) 3~7 days
Wash (PBS) 2 h x3


====Nuclear staining by CUBIC-HV™1 3D nuclear staining kit (Product No. C3698) ====

Nuclear staining (DAPI or Propidium Iodide or others in CUBIC-HV™1 3D nuclear staining buffer) 3~5 days
Wash (3D nuclear staining wash buffer; 10mM HEPES buffer, pH 7.5) 2 h x3


==== Optional process; Enzyme reaction ====


====Immunostaining by CUBIC-HV™1 3D immunostaining kit (Product No. C3708) ====

Primary antibody + secondary antibody reaction / Replacement with CUBIC-HV™1 3D immunostaining buffer 1.5 h
Immunostaining (CUBIC-HV™1 3D immunostaining buffer with CUBIC-HV™1 3D immunostaining additive (if required)) 1 week~
Reaction in the same buffer at 4℃ 1 day
Wash (CUBIC-HV™1 3D immunostaining wash buffer) 2 h x3


====Fix and RI matching====

Post-fix (in 1% Formaldehyde Solution) 1 day
Wash (PBS) 2 h
RI matching (CUBIC-R+; Product No. T3740) ~3 days
Microscopic observation


Related Products

T3740 Tissue-Clearing Reagent CUBIC-L [for Animals]
T3741 Tissue-Clearing Reagent CUBIC-R+ [for Animals]
C3708 CUBIC-HV™1 3D immunostaining kit
F0622 37% Formaldehyde Solution
H0396 HEPES

Product Number C3698
Storage Temperature 0-10°C
Condition to Avoid Heat Sensitive
Specifications
Properties (reference)
GHS
Pictogram Pictogram
Signal Word Warning
Hazard Statements H319 : Causes serious eye irritation.
Precautionary Statements P264 : Wash skin thoroughly after handling.
P280 : Wear eye protection/ face protection.
P337 + P313 : If eye irritation persists: Get medical advice/ attention.
P305 + P351 + P338 : IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
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