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CAS RN: | Product Number: C3698
CUBIC-HV™1 3D nuclear staining kit
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| Size | Unit Price | Saitama (Kawaguchi) | Hyogo (Amagasaki) | Stock in other WH |
|---|---|---|---|---|
| Contact Us | Contact Us | Contact Us |
Available Stock: Prompt shipment (for products) in Saitama/Hyogo warehouse. Stock in other WH: 2-3 Business Days
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*TCI frequently reviews storage conditions to optimize them. Please note that the latest information on the storage temperature for the products is described on our website.
* Please contact us if you need further information.
* To send your quote request for bulk quantities, please click on the "Request Bulk Quote" button. Please note that we cannot offer bulk quantities for some products.
*TCI frequently reviews storage conditions to optimize them. Please note that the latest information on the storage temperature for the products is described on our website.
Note:
This product is discontinued. Please use a successor product, CUBIC-HV™1 3D nuclear staining kit (Product Number: C3709).
Please see the detail protocols in CUBICStars’ web site.
https://www.cubicstars.com/cubic-hv/index.html
Constituents in kit (suitable for a brain of an adult mouse)
- CUBIC-HV™1 3D nuclear staining buffer (1x) (for 10 tests)
- DAPI·2HCl (200x) (for 10 tests)
- Propidium Iodide (100x) (for 10 tests)
- 10 x 5 mL tube
Process outline
====Delipidation====
Perfusion fixation and dissection of the mouse brain
Post-fix ~24 h
Wash (PBS) 2 h x3
Delipidation (CUBIC-L; Product No. T3740) 3~7 days
Wash (PBS) 2 h x3
====Nuclear staining by CUBIC-HV™1 3D nuclear staining kit (Product No. C3698) ====
Nuclear staining (DAPI or Propidium Iodide or others in CUBIC-HV™1 3D nuclear staining buffer) 3~5 days
Wash (3D nuclear staining wash buffer; 10mM HEPES buffer, pH 7.5) 2 h x3
==== Optional process; Enzyme reaction ====
====Immunostaining by CUBIC-HV™1 3D immunostaining kit (Product No. C3708) ====
Primary antibody + secondary antibody reaction / Replacement with CUBIC-HV™1 3D immunostaining buffer 1.5 h
Immunostaining (CUBIC-HV™1 3D immunostaining buffer with CUBIC-HV™1 3D immunostaining additive (if required)) 1 week~
Reaction in the same buffer at 4℃ 1 day
Wash (CUBIC-HV™1 3D immunostaining wash buffer) 2 h x3
====Fix and RI matching====
Post-fix (in 1% Formaldehyde Solution) 1 day
Wash (PBS) 2 h
RI matching (CUBIC-R+; Product No. T3740) ~3 days
Microscopic observation
Related Products
T3740 Tissue-Clearing Reagent CUBIC-L [for Animals]T3741 Tissue-Clearing Reagent CUBIC-R+ [for Animals]
C3708 CUBIC-HV™1 3D immunostaining kit
F0622 37% Formaldehyde Solution
H0396 HEPES
| Product Number | C3698 |
| Storage Temperature | 0-10°C |
| Condition to Avoid | Heat Sensitive |
Specifications
Properties (reference)
GHS
| Pictogram |
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| Signal Word | Warning |
| Hazard Statements | H319 : Causes serious eye irritation. |
| Precautionary Statements | P264 : Wash skin thoroughly after handling. P280 : Wear eye protection/ face protection. P337 + P313 : If eye irritation persists: Get medical advice/ attention. P305 + P351 + P338 : IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. |
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Application
3D staining and imaging for large specimens
References
- Versatile whole-organ/body staining and imaging based on electrolyte-gel properties of biological tissues
Articles/Brochures
TCIMAIL
Documents
Safety Data Sheet (SDS)
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Specifications
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Analytical Charts
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Other Documents
Related Products
CUBIC-HV™1 3D nuclear staining kit
| Product # | C3709 |
| CAS RN | |
| Purity / Analysis Method: |