DBC30 (2mg/mL in Dimethyl Sulfoxide) [for Biochemical Research]

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Purity:

Synonyms:

• 7-(Dibutylamino)-3-(1-methyl-1H-benzimidazol-2-yl)-2H-chromen-2-one (2mg/mL in Dimethyl Sulfoxide)

Product Documents:

SDS | Specifications | C of A & Other Certificates | Analytical Charts

General Information | Specifications & Properties | Safety & Regulations | Applications & Literature

Supplemental Product Information

Explanatory notes for this page

Size	Unit Price	Saitama (Kawaguchi)	Hyogo (Amagasaki)	Stock in other WH	Shipping Information
0.1ML	¥25,000	11	It can be shipped from Saitama warehouse on the same day*	Contact Us	Shipping Simulation

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Product Information

• Product Form : DMSO solution
• Excitation / Emission : 403 nm / 468 nm

Description

• DBC30 is a small compound that emits strong fluorescence in hydrophobic environment. It can dye clearly not only cellular lipid droplets but also tissue lipid droplets. It can be used in combination with multiple fluorescent dyes.
  This product was developed by Dr. Toshitada Yoshihara.

Direction for Use

• 1. Dilute DBC30 solution to 40 μg/mL (100 μM) with DMSO, then dilute to 40 ng/mL (100 nM) with medium such as DMEM.
  2. Remove culture medium and wash cells twice with PBS.
  3. Add 100 nM DBC30-containing medium to cells.
  4. Incubate cells at 37 °C for 30 minutes.
  5. Remove medium and wash cells twice with PBS.
  6. Observe stained lipid droplets.

Directions for Use: Normal or fatty liver mouse imaging

• 1. Dilute DBC30 solution to 200 μg/mL (500 μM) with saline containing 10% BSA.
  2. Inject the 100 µL of 500 µM DBC30 stock solution into the tail vein of an eight-week-old mouse under anesthesia 15 minutes before the fluorescence imaging experiment.
  3. Expose the liver of the mouse and observe it.

Storage

• Store at -20 °C and protect from light. Avoid repeated freezing and thawing.

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