Histone Extraction Kit

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Class II Specified Chemical Substance

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Explanatory notes for this page

Size	Unit Price	Saitama (Kawaguchi)	Hyogo (Amagasaki)	Stock in other WH	Shipping Information
1KIT	¥28,000	14	It can be shipped from Saitama warehouse on the same day*	Contact Us	Shipping Simulation

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* Available Stock: Prompt shipment (for products) in Saitama/Hyogo warehouse. Stock In Other WH: Shipment in about 2-3 Business Days from Saitama warehouse. Please contact us if you need further information.
* The displayed price is the unit price and does not include consumption tax. The unit prices displayed are the latest and are subject to change without notice.
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*TCI frequently reviews storage conditions to optimize them. Please note that the latest information on the storage temperature for the products is described on our website.

Product Information

• Components : 10x Lysis Buffer, 1x Histone Extraction Buffer, 1x Neutralization Buffer
• Size : for 100 tests

Description

• Histones are essential proteins found in the nucleus of eukaryotic cells. They bind to DNA, packaging it into chromosomes and regulating gene expression.
• This product can be used to isolate histones from cultured mammalian cells and tissues, supporting epigenetic analysis.

Directions for Use

• 1. Dilute 10x Lysis Buffer with purified water to prepare 1x Lysis Buffer.
  2. Collect cells via centrifugation and remove supernatant. Use a cell scraper to detach adherent cells.
  3. Resuspend cells in cold 1x Lysis Buffer, adding 1 mL for every 1 x 10⁷ cells.
  4. Incubate on ice for 10 minutes.
  5. Centrifuge at 3,000 x g for 5 minutes at 4 °C and remove the supernatant.
  6. Resuspend the pellet in cold 1x Histone Extraction Buffer. Use 1/10 the amount of buffer used in step 3.
  7. Incubate on ice for 30 minutes.
  8. Centrifuge at 12,000 x g for 5 minutes at 4 °C.
  9. Transfer the supernatant (containing histone) to a new tube.
  10. Add the same amount of 1x Neutralization Buffer as the 1x Histone Extraction Buffer used in step 6.

Storage

• Store at 4 °C.