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CAS RN: | Product Number: I1265
Intracellular Reactive Oxygen Species (ROS) Detection Assay Kit
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| Size | Unit Price | Saitama (Kawaguchi) | Hyogo (Amagasaki) | Stock in other WH |
Shipping Information
|
|---|---|---|---|---|---|
| 1KIT |
¥16,200
|
≥40 | It can be shipped from Saitama warehouse on the same day* | Contact Us |
* Available Stock: Prompt shipment (for products) in Saitama/Hyogo warehouse. Stock In Other WH: Shipment in about 2-3 Business Days from Saitama warehouse. Please contact us
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* The displayed price is the unit price and does not include consumption tax. The unit prices displayed are the latest and are subject to change without notice.
* To send your quote request for bulk quantities, please click on the "Request Bulk Quote" button. Please note that we cannot offer bulk quantities for some products.
*TCI frequently reviews storage conditions to optimize them. Please note that the latest information on the storage temperature for the products is described on our website.
Supplemental Product Information:
Product Information
- Size : 96 tests
Description
- Reactive Oxygen Species (ROS) are mainly produced by mitochondria, regulating processes necessary for cell proliferation, including in cancer cells. Cell death, such as apoptosis and ferroptosis, is associated with an increased intracellular ROS level.1) DCFH-DA is enzymatically deacetylated and hydrolyzed to produce DCFH, which penetrates the cell. Intracellular DCFH is then oxidized to DCF by reactive oxygen species (e.g., OH, O2-, H2O2, etc.), resulting in a strong fluorophoric response that turns the solution greenish-yellow in color.2) This kit is designed to directly visualize the real-time production of activated oxygen species in live cells using fluorescence microscopy equipped with a standard green filter set (450-490 nm / 500-550 nm), or flow cytometry equipped with a blue laser (488 nm / 520 nm).
Components
- 1000X Reaction Dye (DCFH-DA) : 10 μL
- 10X Reaction Buffer : 25 mL×2
Preparation
- 1X Reaction Buffer : Reconstitute the 10X Reaction Buffer by distilled water.
- 1X Reaction Dye : Reconstitute the 1000X Reaction Dye by 1X Reaction Buffer.
Step–by–Step Protocol
Storage
- Stock the 10X Reaction Buffer and 1X Reaction Buffer at 4 °C. Stock the 1000X Reaction dye at -20 °C. Immediately use up the 1X Reaction Dye after reconstitution.
References
- 1) Mitochondrial ROS regulation of proliferating cells
- 2) Time-dependent interactions of oxidant-sensitive fluoroprobes with inhibitors of cellular metabolism
| Product Number | I1265 |
| Physical State (20 deg.C) | Liquid |
Storage Temperature 
|
Frozen (-20°C) |
| Condition to Avoid | Heat Sensitive |
Specifications
Properties (reference)
GHS
Related Laws:
| Fire Defense Law | Group-4-3-III |
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