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CAS RN: | 제품번호: C3708
CUBIC-HV™1 3D immunostaining kit
|포장단위||가격||당일출고||2-3일 이내 출고|
•본건의 원가격은 한국 대리점의 예상 판매가격입니다.자세한 정보가 필요하시면 연락해 주십시오.
(SEJIN CI (한국총대리점) 전화 : 02-2655-2480 이메일 : email@example.com)
This kit, a successor product of CUBIC-HV™1 3D immunostaining kit (Product Number: C3699) contains buffers and anti-NeuN antibody (positive control) for 3D nuclear staining of CUBIC-cleared tissues.
Please see the detail protocols in CUBICStars’ web site.
Constituents in kit (suitable for a brain of an adult mouse)
- CUBIC-HV™1 3D immunostaining buffer (2x) (for 10 tests)
- CUBIC-HV™1 3D immunostaining wash buffer (1x) (for 10 tests)
- CUBIC-HV™1 3D immunostaining additive (10x) (for 10 tests)
- Anti-NeuN Mouse IgG1 antibody (1mg/mL) (for 2 tests)
- 10x 15 mL tube
Perfusion fixation and dissection of the mouse brain
Post-fix <24 h
Wash (PBS) 2 h x3
Delipidation (CUBIC-L; Product No. T3740) 3-7 days
Wash (PBS) 2 h x3
====Nuclear staining by CUBIC-HV™1 3D nuclear staining kit (Product No. C3709) ====
Nuclear staining (DAPI or Propidium Iodide or others in CUBIC-HV™1 3D nuclear staining buffer) 3-5 days
Wash (3D nuclear staining wash buffer; 10mM HEPES buffer, pH 7.5) 2 h x3
==== Optional process; Enzyme reaction ====
====Immunostaining by CUBIC-HV™1 3D immunostaining kit (Product No. C3708) ====
Primary antibody (anti NeuN antibody in C3708 kit can be used): 5 µg/whole brain
Secondary antibody: anti-Fc Fab (out of the kits), 3.75 µg/whole brain
Primary antibody + secondary antibody reaction / Replacement with CUBIC-HV™1 3D immunostaining buffer, 1.5 h
Immunostaining (CUBIC-HV™1 3D immunostaining buffer with CUBIC-HV™1 3D immunostaining additive (if required)), >1 week
Reaction in the same buffer at 4°C, 1 day
Wash (CUBIC-HV™1 3D immunostaining wash buffer), 2 h x3
====Fix and RI matching====
Post-fix (in 1% Formaldehyde Solution), 1 day
Wash (PBS) 2 h
RI matching (CUBIC-R+; Product No. T3740), <3 days
T3740 Tissue-Clearing Reagent CUBIC-L [for delipidation and decoloring]
T3741 Tissue-Clearing Reagent CUBIC-R+ [for RI matching]
C3709 CUBIC-HV™1 3D nuclear staining kit
F0622 37% Formaldehyde Solution
|피해야 할 조건||Heat Sensitive|
|위험물 및 유해 등록||H319 : Causes serious eye irritation.
|주의 사항||P264 : Wash skin thoroughly after handling.
P280 : Wear eye protection/ face protection.
P337 + P313 : If eye irritation persists: Get medical advice/ attention.
P305 + P351 + P338 : IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- Versatile whole-organ/body staining and imaging based on electrolyte-gel properties of biological tissues
- E. A. Susaki, C. Shimizu, A. Kuno, K. Tainaka, X. Li, K. Nishi, K. Morishima, H. Ono, K. L. Ode, Y. Saeki, K. Miyamichi, K. Isa, C. Yokoyama, H. Kitaura, M. Ikemura, T. Ushiku, Y. Shimizu, T. Saito, T. C. Saido, M. Fukayama, H. Onoe, K. Touhara, T. Isa, A. Kakita, M. Shibayama, H. R. Ueda, Nat. Commun. 2020, 11, 1982.
기사 / 브로셔
요청한 SDS를 사용할 수 없습니다.
번거롭게 해드려 죄송하지만 이 양식 보다 문의 해주십시오.
시험성적서， 각종 증명서
죄송합니다만 찾으시는 분석차트는 없습니다.