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CAS RN: | 제품번호: C3708

CUBIC-HV™1 3D immunostaining kit


순도/분석 방법:
동의어의:
문서:
•본 제품의 재고는 일본 본사의 재고입니다. 주문시, 5일안에 실험실까지 도착됩니다.
•본건의 원가격은 한국 대리점의 예상 판매가격입니다.자세한 정보가 필요하시면 연락해 주십시오.
(SEJIN CI (한국총대리점) 전화 : 02-2655-2480 이메일 :   sales@sejinci.co.kr)

Note:

This kit, a successor product of CUBIC-HV™1 3D immunostaining kit (Product Number: C3699) contains buffers and anti-NeuN antibody (positive control) for 3D nuclear staining of CUBIC-cleared tissues.

Please see the detail protocols in CUBICStars’ web site.
https://www.cubicstars.com/cubic-hv/index.html


Constituents in kit (suitable for a brain of an adult mouse)

  • CUBIC-HV™1 3D immunostaining buffer (2x) (for 10 tests)
  • CUBIC-HV™1 3D immunostaining wash buffer (1x) (for 10 tests)
  • CUBIC-HV™1 3D immunostaining additive (10x) (for 10 tests)
  • Anti-NeuN Mouse IgG1 antibody (1mg/mL) (for 2 tests)
  • 10x 15 mL tube

Process outline

====Delipidation====

Perfusion fixation and dissection of the mouse brain
Post-fix <24 h
Wash (PBS) 2 h x3
Delipidation (CUBIC-L; Product No. T3740) 3-7 days
Wash (PBS) 2 h x3


====Nuclear staining by CUBIC-HV™1 3D nuclear staining kit (Product No. C3709) ====

Nuclear staining (DAPI or Propidium Iodide or others in CUBIC-HV™1 3D nuclear staining buffer) 3-5 days
Wash (3D nuclear staining wash buffer; 10mM HEPES buffer, pH 7.5) 2 h x3


==== Optional process; Enzyme reaction ====


====Immunostaining by CUBIC-HV™1 3D immunostaining kit (Product No. C3708) ====

Primary antibody (anti NeuN antibody in C3708 kit can be used): 5 µg/whole brain
Secondary antibody: anti-Fc Fab (out of the kits), 3.75 µg/whole brain
Primary antibody + secondary antibody reaction / Replacement with CUBIC-HV™1 3D immunostaining buffer, 1.5 h
Immunostaining (CUBIC-HV™1 3D immunostaining buffer with CUBIC-HV™1 3D immunostaining additive (if required)), >1 week
Reaction in the same buffer at 4°C, 1 day
Wash (CUBIC-HV™1 3D immunostaining wash buffer), 2 h x3


====Fix and RI matching====

Post-fix (in 1% Formaldehyde Solution), 1 day
Wash (PBS) 2 h
RI matching (CUBIC-R+; Product No. T3740), <3 days
Microscopic observation


Related Products

T3740 Tissue-Clearing Reagent CUBIC-L [for delipidation and decoloring]
T3741 Tissue-Clearing Reagent CUBIC-R+ [for RI matching]
C3709 CUBIC-HV™1 3D nuclear staining kit
F0622 37% Formaldehyde Solution
H0396 HEPES

제품번호 C3708
보관 조건 0-10°C
피해야 할 조건 Heat Sensitive
규격표
물성치(참고치)
GHS
픽토그램 Pictogram
신호 워드 Warning
위험물 및 유해 등록 H319 : Causes serious eye irritation.
주의 사항 P264 : Wash skin thoroughly after handling.
P280 : Wear eye protection/ face protection.
P337 + P313 : If eye irritation persists: Get medical advice/ attention.
P305 + P351 + P338 : IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
법규 정보
운송 정보
HS 번호* 3822.00-000
*이 HS 번호 는 TCI(일본)에서 제품을 수출할 때만 사용됩니다
문서
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