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3D Tissue Staining Kits CUBIC-HV™

CUBIC-HV is a new technology for highly efficient three-dimensional (3D) whole-organ and whole-body staining and imaging, which has been developed by Dr. Ueda, Dr. Susaki and colleagues at RIKEN. CUBICStars, Co. holds the license for CUBIC-HV and manufactures the reagent kits.
TCI offers two CUBIC-HV™1 kits for use in the CUBIC-HV method.

Products

C3709
CUBIC-HV™1 3D nuclear staining kit
Containing buffers and representative nuclear stains to perform 3D nuclear staining of CUBIC-cleared tissues
C3708
CUBIC-HV™1 3D Immunostaining kit
Containing buffers and anti-NeuN antibody (positive control) to perform 3D immunostaining of CUBIC-cleared tissues

*Components of the kits are subject to change without notice.

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Application (for a single adult mouse brain)

  • Delipidation:
      by CUBIC-L [T3740] at 37°C for 2-3 days.
  • 3D nuclear staining:
      by CUBIC-HV™ 1 3D nuclear staining kit [C3709].
      Immerse your sample in CUBIC-HV™ 1 3D nuclear staining buffer containing DAPI, propidium iodide (included in the kit), or your preferred nuclear staining reagent, and incubate it at 37°C for 3-5 days.
  • 3D immunostaining:
      by CUBIC-HV™ 1 3D Immunostaining kit [C3708].
      Immerse your sample in CUBIC-HV™ 1 3D immunostaining buffer containing anti-NeuN antibody (included in the kit) or your preferred antibody, and incubate it at 25-32°C for over one week (dependent on the antibody).
  • Fixation:
      by 1% Formaldehyde Solution at 4°C for 1 day and then at 37°C for 1 hour.
  • RI matching:
      by CUBIC-R+ [T3741] at room temperature for 2-3 days.

*It is recommended to perform 3D nuclear staining followed by 3D immunostaining.

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