DBD-H [=4-(N,N-Dimethylaminosulfonyl)-7-hydrazino-2,1,3-benzoxadiazole] [for HPLC Labeling]

The compound 1 is an HPLC fluorescence labeling reagent, which has a 2,1,3-benzoxadiazole skeleton and a hydrazino group, easily reacts with a carbonyl group to form the corresponding hydrazone. The resultant hydrazone is stable enough to reach the detector without any decomposition under reversed phase HPLC. An excellent chromatogram can be obtained by fluorescence detection at the excitation and emission wavelengths of 450 nm and 565 nm, respectively. Since their excitation and fluorescence wavelengths are at longer wavelengths, detection has less interference by contaminants. A highly sensitive detection can be done because of its strong fluorescence.

Application examples:
[Aldehydes or ketones] ¹⁾
250 µM of the labeling reagent 1 and 1.7 µM propionaldehyde are added to acetonitrile containing 0.025% TFA, and reacted at room temperature for 30 min, then use it as the HPLC sample.
For example, the detection limit for propionaldehyde is 120 fmol.

TCI Products Pamphlet
HPLC Labeling Reagents ( PDF 10MB )