1,3-Cyclohexanedione [for HPLC Labeling]

The compound 1 is an HPLC fluorescence labeling reagent, and can easily react with a carbonyl groups to form the corresponding decahydroacridine-1,8-dion (DHA) derivative. The resultant derivative is stable enough to reach the detector without any decomposition under reversed phase HPLC. An excellent chromatogram can be obtained by fluorescence detection analysis at the excitation and emission wavelengths of 366 nm and 440 nm, respectively.

Application example:
[Aliphatic aldehydes] ^1,2)
5 mL of acetic acid and 10 g of ammonium acetate are dissolved in distilled water. Then 0.25 g of labeling reagent 1 is added to the solution and shaken to prepare the derivatization reagent solution. 2 mL of this solution is added to 1 mL of aqueous solution (ethanol solution, in the case of long-chain aldehydes) containing 10~30 ng of an aliphatic aldehyde, and incubate at 60 °C for 30 min. After cooling, use 1 µL of this solution as an HPLC sample.

TCI Products Pamphlet
HPLC Labeling Reagents ( PDF 10MB )