DiD Solution [for Cell Membrane Staining]

• 

×

×

Reinheit:

Synonyme

• 2-[5-(3,3-Dimethyl-1-octadecylindolin-2-ylidene)penta-1,3-dien-1-yl]-3,3-dimethyl-1-octadecyl-3H-indol-1-ium Perchlorate Solution

Produktdokumente:

SDS | Spezifikation | AZ & andere Zertifikate | Analytische Diagramme

Generelle Informationen | Spezifikationen und Eigenschaften | Sicherheit und Vorschriften | Anwendungen & Literatur

Ergänzende Produktinformation

Erläuterung zu dieser Seite

Einheit	Stückpreis	Belgien	Japan*	Menge	Versandinformationen
1ML	€179.00	Kontaktieren Sie uns	6		Versandsimulation

*Lagerbestand in Belgien: Versand am selben Tag
*Lagerbestand in Japan: Bitte prüfen Sie die Versandsimulation für voraussichtliche Lieferzeiten. (ausgenommen regulierte Produkte und Trockeneissendungen)

Zu Ihrem Warenkorb hinzugefügt

In den Warenkorb legen

Fordern Sie ein BULK-Angebot an

Product Information

• Concentration : 1 mM
• Excitation / Emission : 657 nm / 678 nm

Description

• DiD is a lipophilic carbocyanine dye used to label cell membranes. It emits strong fluorescence when its long hydrocarbon chains are incorporated into a lipid bilayer. This product is a ready-to-use solution of DiD which can be used as a tracer in cell fusion, cell adhesion and chemotaxis.

Storage

• Store at room temperature and protect from light.

Directions for Use: Staining live cells

• 1. Dilute DiD solution to 5 μM with culture medium.
  2. Remove medium from cells and replace with 5 μM DiD-containing medium.
  3. Incubate cells at 37 °C for 10 minutes.
  4. Remove medium and wash cells 3 times with PBS.
  5. Observe stained cell membranes via a fluorescence microscope.

Directions for Use: Staining fixed cells

• 1. Remove culture medium and wash cells twice with PBS.
  2. Add 4% paraformaldehyde to cells and incubate at room temperature for 10 minutes.
  3. Dilute DiD solution to 5 μM with PBS.
  4. Remove 4% paraformaldehyde and add 5 μM DiD-containing PBS to cells.
  5. Incubate cells at 37 °C for 10 minutes.
  6. Remove medium and wash cells 3 times with PBS.
  7. Observe stained cell membranes via a fluorescence microscope.