N-Succinimidyl 4-Nitrophenylacetate [for HPLC Labeling]

The compound 1 is an HPLC labeling reagent, which has a succinimidyl group, which can easily react with an amino group to form the corresponding amide derivative. The resultant amide is stable enough to reach the detector without any decomposition under reversed phase HPLC. An excellent chromatogram can be obtained by measuring at 254 nm, an absorption wavelength that is generally used for UV detection.

Application examples:
[Alkylamines]
1~5 mg of a sample (free amine), 5 mL of THF, and 50 mg of labeling reagent 1 are mixed, and incubated at 60°C for 1 h. After cooling to room temperature, use it as an HPLC sample. If it is necessary to remove the unreacted labeling reagent and by-product, N-hydroxysuccinimide, evaporate the solvent at a low temperature under a nitrogen atmosphere. Dissolve the residue in 2~3 mL of ether and wash with aqueous NaHCO₃ and water.
[Others]
Drugs (amphetamine, methamphetamine)¹⁾

TCI Products Pamphlet
HPLC Labeling Reagents ( PDF 10MB )