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CAS RN: | Product Number: R0233
rSRL-Biotin [for GlcNAcβ(1-2)Man, Galβ(1-3)GalNAc]
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Purity:
Synonyms:
- rSRL-Biotin Conjugate
- Recombinant Sclerotium rolfsii lectin (= rSRL)-Biotin Conjugate
Product Documents:
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Supplemental Product Information:
Product Information
Product code: R0233
Product name: rSRL-Biotin [for GlcNAcβ(1-2)Man, Galβ(1-3)GalNAc]
Quantity: 1 ml per vial (1 mg/1 ml: Lowry method)
Product form: Biotin-immobilized recombinant SRL, Liquid
Buffer: Phosphate buffered saline [PBS] (-), pH7.4
Molecular Mass: >16 kDa
Preservative : None
Stabilizer: None
Description
The rSRL is a carbohydrate-binding protein (Lectin) derived from Sclerotium rolfsii. The rSRL gene product is expressed in Escherichia coli. This lectin preferably binds to two types [GlcNAcβ(1-2)Man, Galβ(1-3)GalNAc] of glycan epitopes by its two distinct carbohydrate-binding sites.
Biotin is covalently linked to the protein surface. Resulting band shift caused by biotinylation is checked by SDS-PAGE. (Unbiotinylated band is mostly not observed.) The rSRL-Biotin shows significant interaction with agalacto human transferrin (hTF) possessing [GlcNAcβ(1-2)Man] glycan epitopes by lectin-ELISA analysis. According to the lectin-blotting analysis, the rSRL-Biotin specifically binds to β(1-2)GlcNAc-terminated hTF but not to non-β(1-2)GlcNAc-terminated glycoproteins such as hTF, asialo hTF and bovine RNase B.
Storage
Store at -20°C to -80°C. Avoid repetitive freezing and thawing. After thawing the product, it’s recommended to store at 2-10°C with an additional preservative (e.g., 0.02-0.05% [w/v] sodium azide).
Related Products
R0228 Recombinant Sclerotium rolfsii lectin (= rSRL) expressed in Escherichia coli
R0238 rSRL-LecBeads [for GlcNAcβ(1-2)Man, Galβ(1-3)GalNAc]
R0230 rPSL1a-Biotin [for Sia α(2-6)Gal]
R0231 rLSL-N-Biotin [for Galβ(1-4)GlcNAc, poly LacNAc]
R0232 rMOA-Biotin [for Galα(1-3)Gal]
R0234 rGRFT-Biotin [for Manα(1-2)Man]
A2659 AOL-Biotin Conjugate
| Product Number | R0233 |
| Physical State (20 deg.C) | Liquid |
Storage Temperature 
|
Frozen (-20°C) |
| Condition to Avoid | Heat Sensitive |
Specifications
| Appearance | Colorless to Almost colorless clear liquid |
| Concentration(Lowry method) | 0.9 to 1.5 mg/mL |
| Molecular weight | 10 to 25 kDa |
| Suitability for use in ELISA tests | min. 1.0 (10 micro g/mL, OD450) |
Properties (reference)
GHS
Related Laws:
Transport Information:
| H.S.code* | 3504.00-000 |
Application
Recombinant lectin
References
- A newly isolated lectin from the plant pathogenic fungus Sclerotium rolfsii: purification, characterization and role in mycoparasitism
- Carbohydrate specificity of a lectin isolated from the fungus Sclerotium rolfsii
- Structural basis for the carbohydrate recognition of the Sclerotium rolfsii lectin
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Other Documents
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rSRL-LecBeads [for GlcNAcβ(1-2)Man, Galβ(1-3)GalNAc]
| Product # | R0238 |
| CAS RN | |
| Purity / Analysis Method: |
Recombinant Sclerotium rolfsii lectin (= rSRL) expressed in Escherichia coli
| Product # | R0228 |
| CAS RN | |
| Purity / Analysis Method: |
N-Acetyl-D-glucosamine
| Product # | A0092 |
| CAS RN | 7512-17-6 |
| Purity / Analysis Method: | >98.0%(HPLC)(N) |