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CAS RN: 1239-45-8 | Product Number: E0370
Ethidium Bromide
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Purity: >95.0%(T)(HPLC)
Synonyms:
- 3,8-Diamino-5-ethyl-6-phenylphenanthridinium Bromide
- Homidium Bromide
Product Documents:
| Size | Unit Price | Belgium | Japan* | Quantity |
Shipping Information
|
|---|---|---|---|---|---|
| 1G |
€82.00
|
3 | ≥40 |
|
|
| 5G |
€320.00
|
1 | 7 |
|
* Stock available in Belgium: Shipment on the same day
* Stock available in Japan: Please check the Shipping Simulation for estimated shipments. (excludes regulated items and dry ice shipments)
| Product Number | E0370 |
Purity / Analysis Method 
|
>95.0%(T)(HPLC) |
| Molecular Formula / Molecular Weight | C__2__1H__2__0BrN__3 = 394.32 |
| Physical State (20 deg.C) | Solid |
| Storage Temperature
|
Room Temperature (Recommended in a cool and dark place, <15°C) |
| Packaging and Container
|
1G-Glass Bottle with Plastic Insert (View image) |
| CAS RN | 1239-45-8 |
| Reaxys Registry Number | 3642536 |
| PubChem Substance ID | 87569630 |
| SDBS (AIST Spectral DB) | 13171 |
| Merck Index (14) | 4731 |
| MDL Number | MFCD00011724 |
Specifications
| Appearance | Orange to Brown to Dark purple powder to crystal |
| Purity(HPLC) | min. 95.0 area% |
| Purity(Argentometric Titration) | min. 95.0 % |
Properties (reference)
GHS
| Pictogram |
|
| Signal Word | Danger |
| Hazard Statements | H330 : Fatal if inhaled. H302 : Harmful if swallowed. H315 : Causes skin irritation. H319 : Causes serious eye irritation. H341 : Suspected of causing genetic defects. |
| Precautionary Statements | P260 : Do not breathe dust. P201 : Obtain special instructions before use. P264 : Wash skin thoroughly after handling. P280 : Wear protective gloves/ protective clothing/ eye protection/ face protection/ hearing protection. P304 + P340 + P310 : IF INHALED: Remove person to fresh air and keep comfortable for breathing. Immediately call a POISON CENTER/doctor. P403 + P233 : Store in a well-ventilated place. Keep container tightly closed. |
Related Laws:
| EC Number | 214-984-6 |
| RTECS# | SF7950000 |
Transport Information:
| UN Number | UN1544 |
| Class | 6.1 |
| Packing Group | I |
| HS Number | 2933998090 |
Application
Efflux pump activity assay
Ethidium bromide is used to measure drug efflux activities.1,2)
References
- 1) Phenotype microarray analysis of the drug efflux systems in Salmonella enterica serovar Typhimurium
- 2) Functional cloning and expression of emeA, and characterization of EmeA, a multidrug efflux pump from Enterococcus faecalis
Application
Ethidium Bromide: A Widely Used Dye for DNA and RNA Detection
Ethidium bromide is widely used for DNA and RNA detection. Ethidium bromide binds to double-stranded DNA as an intercalator, inserting itself between the base pairs in the double helix, resulting in changes in the absorption and fluorescence properties of the dye. Ethidium bromide also is used to detect protein–DNA complexes.
References
- A fluorescent complex between ethidium bromide and nucleic acids
- Mechanism of ethidium bromide fluorescence enhancement on binding to nucleic acids
- Mechanism of intercalation into the DNA double helix by ethidium
- Ethidium bromide provides a simple tool for identifying genuine DNA-independent protein associations
- Rapid and sensitive ethidium bromide fluorescence quenching assay of polyamine conjugate-DNA interactions for the analysis of lipoplex formation in gene therapy
- Stability and structural features of DNA intercalation with ethidium bromide, acridine orange and methylene blue
PubMed Literature
Product Documents (Note: Some products will not have analytical charts available.)
Safety Data Sheet (SDS)
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Specifications
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Other Documents
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Ethidium Bromide (0.5mg/mL in Water) (in Dropper Bottle) [for Electrophoresis]
| Product # | E1363 |
| CAS RN | 1239-45-8 |
| Purity / Analysis Method: |