DiD Solution [for Cell Membrane Staining]

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Synoniemen

• 2-[5-(3,3-Dimethyl-1-octadecylindolin-2-ylidene)penta-1,3-dien-1-yl]-3,3-dimethyl-1-octadecyl-3H-indol-1-ium Perchlorate Solution

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Afmeting	Eenheidsprijs	België	Japan*	Hoeveelheid	Verzendinformatie
1ML	€179.00	Neem contact met ons op	19		Simulatie van verzending

*Voorraad beschikbaar in België: Verzending dezelfde dag
*Voorraad beschikbaar in Japan: Raadpleeg de verzendsimulatie voor geschatte levertijden. (met uitzondering van gereguleerde producten en zendingen met droogijs)

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Product Information

• Concentration : 1 mM
• Excitation / Emission : 657 nm / 678 nm

Description

• DiD is a lipophilic carbocyanine dye used to label cell membranes. It emits strong fluorescence when its long hydrocarbon chains are incorporated into a lipid bilayer. This product is a ready-to-use solution of DiD which can be used as a tracer in cell fusion, cell adhesion and chemotaxis.

Storage

• Store at room temperature and protect from light.

Direction for Use

• Staining live cells

  1. Dilute DiD solution to 5 μM with culture medium.
  2. Remove medium from cells and replace with 5 μM DiD-containing medium.
  3. Incubate cells at 37 °C for 10 minutes.
  4. Remove medium and wash cells 3 times with PBS.
  5. Observe stained cell membranes via a fluorescence microscope.

• Staining fixed cells

  1. Remove culture medium and wash cells twice with PBS.
  2. Add 4% paraformaldehyde to cells and incubate at room temperature for 10 minutes.
  3. Dilute DiD solution to 5 μM with PBS.
  4. Remove 4% paraformaldehyde and add 5 μM DiD-containing PBS to cells.
  5. Incubate cells at 37 °C for 10 minutes.
  6. Remove medium and wash cells 3 times with PBS.
  7. Observe stained cell membranes via a fluorescence microscope.

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