Tissue-Clearing Reagent iTOMEI-M (RI 1.40) [for Plants]

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Afmeting	Eenheidsprijs	België	Japan*	Hoeveelheid
5ML	€27.00	1	14
25ML	€74.00	4	34

*Stock beschikbaar uit voorraad in België leverbaar in 1 tot 3 dagen
*stock beschikbaar uit voorraad in Japan leverbaar in 1 tot 2 weken (met uitzondering van gereguleerde producten en zendingen met droog ijs)

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"Improved TOMEI (iTOMEI)" is a plant tissue-clearing method that specializes TOMEI for fluorescent protein observation by Prof. Sakamoto et al. iTOMEI enables simply transparency and clearly detecting fluorescent protein.
This product can be used as a mounting agent in iTOMEI. Its refractive index is 1.40, optimized to silicone immersion objectives.

Please see a product brochure (PDF) of how to use.

Description

This product enables transparency by simple osmosis in just a few days (more than 2 days) and keeps the fluorescence of a fluorescent protein such as GFP or tdTomato co-expressed as a reporter gene. The refractive index (RI) of this product is optimized to silicone immersion objectives.
The decoloring solution is also required to complete the iTOMEI method.

Storage

The product should be stored at 4 °C.

Directions for Use

Required reagents

• Paraformaldehyde (PFA)
• PBS
• Stains* (option)

==Process outline (All operations are performed at Room temperature) ===============

• Tissue fixation
  Reagent : 1% PFA in PBS
  Time : 1hr
• PBS Wash ×3
• Decoloring
  Reagent : iTOMEI-D (Product No. T3940)
  Time : 24hr
• PBS Wash ×3
• (Optional process Staining*, PBS Wash ×3)
• Clearing (RI matching)**
  Reagent : iTOMEI-M (Product No. T4003)
  Time : Operation time is determined by the mounting solution.
• Mounting**
  Reagent : iTOMEI-M (Product No. T4003)

*The treatment time of DAPI staining is for 30min with 5 µg/mL and the treatment time of Calcofluor White staining is for 10min with 1 g/L of Calcofluor White M3R and 0.5 g/L of Evans Blue, but their adjustments are needed for the purpose.
** Please use the suitable mounting solution for objective lens.
(iTOMEI-M is optimized to silicone immersion objectives.)

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