Published TCIMAIL newest issue No.200
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Biotin Quantitation Kit
HABA (= 4'-Hydroxyazobenzene-2-carboxylic Acid) reversibly binds to the biotin-binding site of avidin, absorbing light around 500 nm. When biotin is added, it displaces HABA, causing a decrease in absorbance. This change can be used to determine the amount of biotin present.
Biotin Quantitation Kit (Product No. B7077) contains HABA solution, avidin solution, and a biotin standard solution. The kit can be used to determine the biotin labeling rate of biotin-protein complexes or quantify biotin concentration in solution.
Product
Biotins and Streptavidins
The avidin-biotin system is widely used for bioanalysis and bioassays including flow cytometry, ELISA, immunohistochemical staining, western blotting and others.
Biotin labeling (biotinylation) is also commonly used for conjugating proteins, especially antibodies, and other various molecules. Biotinylation is one of the most essential methods in the field of immunoassay where antigens are detected using antibodies. Streptavidin is a protein from the avidin family having extraordinarily high affinity for biotin, in fact, the interaction of biotin with streptavidin is among the strongest non-covalent affinities known in nature. In order to detect the biotinylated substance, modification of streptavidin with fluorescent label or enzyme is required.
The biotinylated substance and the labeled-streptavidin are used in various assays based on the avidin-biotin system.
Products (Biotinylation Reagents)
Biotinylation Reagents for Amino Groups
- B0463
- Biotin
- S0491
- D-Biotin N-Succinimidyl Ester
- B3173
- Biotin Pentafluorophenyl Ester
- B4009
- (+)-Biotin 4-Nitrophenyl Ester
- B2433
- 6-Biotinamidohexanoic Acid
- S0490
- 6-Biotinamidohexanoic Acid N-Succinimidyl Ester
- B5711
- Biotin-PEG3-acetic Acid
- B5562
- Biotin-PEG4-propionic Acid
- B6207
- Biotin-LC-LC
- S0955
- Biotin-PEG2-NHS
- S0956
- Biotin-LC-LC-NHS
- U0106
- Sulfo-NHS-LC-LC-Biotin
Biotinylation Reagents for Carboxy Groups, Aldehydes and Ketones
Biotinylation Reagents for Aldehydes and Ketones
Biotinylation Reagents for Thiol Groups
Biotinylation Reagents for Click Reactions
- A2524
- Biotin-azide
- A2523
- Biotin-PEG3-Azide
- B5546
- Biotin-PEG4-Azide
- B6602
- Biotin Alkyne
- B6511
- Biotin-PEG4-Alkyne
- D5552
- Dibenzocyclooctyne-S-S-PEG3-Biotin
- T4237
- TCO-PEG3-Biotin equatorial isomer
- T4259
- TCO*-PEG3-Biotin equatorial isomer
- T4280
- Tetrazine-amido-PEG3-Biotin
- M3768
- Methyltetrazine-amido-PEG3-Biotin
Biotinylation Reagents for Others
Products (Desthiobiotinylation Reagents)
- H1667
- Hydrazide-PEG4-Desthiobiotin (*This product is discontinued.)
- A3202
- Azide-PEG3-Desthiobiotin
Products (Pre-Weighed Biotinylation Reagents)
Pre-Weighed Biotinylation Reagents for Amino Groups
Pre-Weighed Biotinylation Reagents for Carboxy Groups
Pre-Weighed Biotinylation Reagents for Click Reactions
Products (Streptavidin and Labeled Streptavidins)
Products (Biotin-labeled Antibodies)
- A3144
- Anti-αGal Polyclonal Antibody Biotin Conjugate
- A2822
- Anti-Gb3 Monoclonal Antibody Biotin Conjugate
- A3226
- Anti-GST Monoclonal Antibody Biotin Conjugate
- A3294
- Anti-NeuGc Polyclonal Antibody Biotin Conjugate
- A3045
- Anti-Protein A Chicken Polyclonal Antibody Biotin Conjugate
- A3010
- Anti-6xHis Monoclonal Antibody (6A12) Biotin Conjugate
- G0387
- Goat Anti-Mouse IgG Biotin Conjugate
- G0432
- Goat Anti-Mouse IgM Biotin Conjugate
- G0597
- Goat Anti-Rabbit IgG Biotin Conjugate
- M3400
- Mouse Anti-DYKDDDDK Monoclonal Antibody Biotin Conjugate
- M3053
- Mouse Anti-Human IgG Fc Biotin Conjugate
- H1619
- Sheep Anti-Chicken IgY Biotin Conjugate