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CAS RN: 2086-26-2 | Product Number: A5532
O-4-Nitrobenzylhydroxylamine Hydrochloride [for HPLC Labeling]
![Thumbnail of O-4-Nitrobenzylhydroxylamine Hydrochloride [for HPLC Labeling]](/medias/A5532.jpg?context=bWFzdGVyfHJvb3R8MTU4MTF8aW1hZ2UvanBlZ3xhR0UxTDJnd01TODRPVE16TVRneU1qYzFOakUwTDBFMU5UTXlMbXB3Wnd8MWNkNTFkYmMxODhjODQ4N2MxN2FlODI2Mzc1MDZlMzA0Mjc0ZjExZjU3ODM4YTgzNmQ1Y2ZhYTJhNDY0ZmM4Mg "O-4-Nitrobenzylhydroxylamine Hydrochloride [for HPLC Labeling]")
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Purity: >99.0%(HPLC)
Synonyms:
Product Documents:
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Shipping Information
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|---|---|---|---|---|---|
| 1G |
$57.00
|
≥40 | 0 | Contact Us | |
| 5G |
$196.00
|
2 | 19 | Contact Us |
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| Product Number | A5532 |
Purity / Analysis Method 
|
>99.0%(HPLC) |
| Molecular Formula / Molecular Weight | C__7H__8N__2O__3·HCl = 204.61 |
| Physical State (20 deg.C) | Solid |
| Storage Temperature
|
Room Temperature (Recommended in a cool and dark place, <15°C) |
| Store Under Inert Gas | Store under inert gas |
| Condition to Avoid | Air Sensitive,Hygroscopic |
| CAS RN | 2086-26-2 |
| Reaxys Registry Number | 3709565 |
| PubChem Substance ID | 87563167 |
| MDL Number | MFCD00012954 |
Specifications
| Appearance | White to Light yellow to Light orange powder to crystal |
| Purity(HPLC) | min. 99.0 area% |
| Purity(Argentometric Titration) | min. 98.0 % |
| Solubility in hot Water | almost transparency |
| Effect test of HPLC derivatization | Effective as labeling agent for Valeraldehyde |
Properties (reference)
| Melting Point | 210 °C(dec.) |
GHS
Related Laws:
| RTECS# | NC4400050 |
Transport Information:
| H.S.code* | 2928.00-000 |
Application
HPLC Labeling Reagent for Carbonyl Compounds
The compound 1 is an HPLC labeling reagent, which has a hydroxylamino moiety, can easily react with a carbonyl group to form the corresponding oxime. The resultant oxime is stable enough to reach the detector without any decomposition under reversed phase HPLC. An excellent chromatogram can be obtained by measuring at 254 nm, an absorption wavelength that is generally used for UV detection.
Application examples:
[Aldehydes] 1)
1~5 mg of a sample, 4 mL of methanol, 2 drops of triethylamine, and 40 mg of the labeling reagent 1 are mixed. Close the cap of the reaction vessel and incubate the mixture at 65 °C for 1 h. After cooling to room temperature, use it as the HPLC sample solution. If it is necessary to remove the unreacted labeling reagent and triethylamine, evaporate the solvent at a low temperature under a nitrogen atmosphere. Dissolve the residue in 2~3 mL of ether and wash with diluted HCl and water.
[Aldehydes] 1)
1~5 mg of a sample, 4 mL of methanol, 2 drops of triethylamine, and 40 mg of the labeling reagent 1 are mixed. Close the cap of the reaction vessel and incubate the mixture at 65 °C for 1 h. After cooling to room temperature, use it as the HPLC sample solution. If it is necessary to remove the unreacted labeling reagent and triethylamine, evaporate the solvent at a low temperature under a nitrogen atmosphere. Dissolve the residue in 2~3 mL of ether and wash with diluted HCl and water.
TCI Products Pamphlet
HPLC Labeling Reagents ( PDF 10MB )
HPLC Labeling Reagents ( PDF 10MB )
References
- 1)T. H. Jupille, Am. Lab. 1976, 8, 85.
PubMed Literature
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