Organosilica Rhodamine B (100nm Diam.)

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Explanatory notes for this page

Size	Unit Price	Same Day	2-3 Business Days	Other Lead Time	Shipping Information
2MG	$182.00	29	0	Contact Us	Shipping Simulation

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* The storage conditions are subject to change without notice.

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Product Information

• Volume/Quantity : 2 mg
• Product Form : Suspension in water
• Functional Group : Thiol group (-SH)
• Excitation / Emission : 556 nm / 579 nm

Application

• For use as a fluorophore in the fluorescent labeling of proteins and other molecules. Incorporation of Rhodamine B fluorescent dye into conjugatable silica nanoparticles allows for superior fluorescence intensity and light resistance when compared to unmodified Rhodamine B. Thiol groups (-SH) at the surface of the nanoparticles provide a convenient mechanism for conjugation. This product is made without toxic metals.

Storage

• Store at 4 °C. Do not freeze.

Direction for Use

• 1. Prepare 10 mM linker solution (For examples of linkers, see Related Products).
     e.g. Dissolve 2.8 mg of GMBS (Product No. S0399) in 1 mL of DMSO (Product No. D0798).
  2. Mix 10 mM linker solution with appropriate volume of antibody. (Suggested linker:antibody molar ratio = 100:1)
     e.g. Mix 100 µL of 1.5 mg/mL (= 10 µM) antibody solution with 10 µL of 10 mM linker solution.
  3. Incubate linker-antibody solution for 1 hour at room temperature.
  4. Remove unreacted and/or hydrolyzed reagent using a desalting column or dialysis method (not included).
  5. Mix 1 part by weight purified antibody-linker solution with 1.5 parts by weight Organosilica Rhodamine B (Product No. O0573).
     e.g. For the antibody-linker solution described in step 2 (antibody weight approx. 0.15 mg), use 45 µL of 5.0 mg/mL Organosilica Rhodamine B.
  6. Incubate the antibody-nanoparticle solution for 1 hour at room temperature.
  7. Collect conjugated nanoparticles via centrifugation (20,000 x g, 4 °C, 10 minutes) and wash with 500 µL deionized water. Repeat wash step two additional times. Resuspend final washed pellet in deionized water.

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