BPMP-2Thia (5mM in Dimethyl Sulfoxide) [for Biochemical Research]

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Size	Unit Price	Same Day	2-3 Business Days	Other Lead Time	Shipping Information
0.1ML	$228.00	0	0	It can be shipped in about 2-3 business days after ordering	Shipping Simulation

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* The storage conditions are subject to change without notice.

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Product Information

• Product Form : DMSO solution
• Excitation / Emission : 599 nm / 636 nm (in Dibutyl Ether)

Description

• BPMP-2Thia is a small compound that emits strong fluorescence in hydrophobic environment. It can dye clearly not only cellular lipid droplets but also tissue lipid droplets. It can be used in combination with multiple fluorescent dyes.
• This product was developed by Dr. Toshitada Yoshihara.

Direction for Use

• Lipid cell imaging

  1. Dilute BPMP-2Thia solution to 1 mM with DMSO, then dilute to 500 nM with medium such as DMEM.
  2. Remove culture medium and wash cells twice with PBS.
  3. Add 500 nM BPMP-2Thia-containing medium to cells.
  4. Incubate cells at 37 °C for 30 minutes.
  5. Remove medium and wash cells twice with PBS.
  6. Observe stained lipid droplets.

• Normal or fatty liver mouse imaging

  1. Dilute BPMP-2Thia solution to 500 μM with saline containing 10% BSA.
  2. Inject the 100 µL of 500 µM BPMP-2Thia stock solution into the tail vein of eight-week-old mouse under anesthesia 15 minutes before the fluorescence imaging experiment.
  3. Expose the liver of the mouse and observe it.

Storage

• Store at −20 °C and protect from light. Avoid repeated freezing and thawing.

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