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CAS RN: | Product Number: R0225
Recombinant Polyporus squamosus lectin (= rPSL1a) expressed in Escherichia coli
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[Handling Notes]
Avoid multiple freeze-thaw cycles. Add some preservative, e.g. sodium azide upon opening for the first time, and store at 2-10 °C.
Product Information
Product code: R0225
Product name: Recombinant Polyporus squamosus lectin (=rPSL1a) expressed in Escherichia coli
Quantity: 1 ml per vial (1 mg/1 ml: Lowry method)
Product form: Purified recombinant PSL1a, Liquid
Buffer: Phosphate buffered saline [PBS] (-), pH7.4
Molecular Mass: 31 kDa
Preservative : None
Stabilizer: None
Description
The rPSL1a is a carbohydrate-binding protein (Lectin) derived from Polyporus squamosus. The rPSL1a gene product is expressed in Escherichia coli. This lectin preferably binds to a [Siaα(2-6)Gal] glycan epitope (It very slightly interacts with lactose.). Consequently, rPSL1a exhibits a hemagglutination activity to a rabbit reticulocyte through the glycan binding to subsites and subunits of the lectin.
Storage
Store at -20°C to -80°C. Avoid repetitive freezing and thawing. After thawing the product, it’s recommended to store at 2-10°C with an additional preservative (e.g., 0.02-0.05% [w/v] sodium azide).
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R0235 rPSL1a-LecBeads [for Siaα(2-6)Gal]
R0226 Recombinant Laetiporus sulphureus lectin N-Terminal Domain (= rLSL-N) expressed in Escherichia coli
R0227 Recombinant Marasmius oreades agglutinin (= rMOA) expressed in Escherichia coli
R0228 Recombinant Sclerotium rolfsii lectin (= rSRL) expressed in Escherichia coli
R0229 Recombinant Griffithsia sp. lectin (= rGRFT) expressed in Escherichia coli
L0169 Lectin, Fucose specific (= AOL) from Aspergillus oryzae (5mg/mL, PBS pH6.5)
| Product Number | R0225 |
| Physical State (20 deg.C) | Liquid |
Storage Temperature 
|
Frozen (-20°C) |
| Condition to Avoid | Heat Sensitive |
Specifications
| Appearance | Colorless to Light yellow to Light orange clear liquid |
| Concentration(Lowry method) | gt. 0.5 to 1.5 mg/mL |
| Molecular weight | 25000 to 40000(SDS-PAGE) |
| Hemagglutination activity | max. 12.5 micro g/mL(pH7.2) |
Properties (reference)
GHS
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Transport Information:
| H.S.code* | 3504.00-000 |
Application
Recombinant lectin
References
- Purification and characterization of a Neu5Acα2-6Galβ1-4Glc/GlcNAc-specific lectin from the fruiting body of the polypore mushroom Polyporus squamosus
- Cloning, expression in Escherichia coli and characterization of the recombinant Neu5Acα2,6Galβ1,4GlcNAc-specific high-affinity lectin and its mutants from the mushroom Polyporus squamosus
- Structure and binding analysis of Polyporus squamosus lectin in complex with the Neu5Acα2-6Galβ1-4GlcNAc human-type influenza receptor
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| Product # | R0235 |
| CAS RN | |
| Purity / Analysis Method: |