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CAS RN: 1621579-96-1 | Product Number: C4223
Coumarin Tyramide 351 [for Tyramide Signal Amplification] (50 tests)
![Thumbnail of Coumarin Tyramide 351 [for Tyramide Signal Amplification] (50 tests)](/medias/Tci-98-C4223.jpg?context=bWFzdGVyfHJvb3R8MjAzMHxpbWFnZS9qcGVnfGFHWTJMMmc1TVM4NU5qWTJOekEzTXprd05EazBMMVJqYVMwNU9GOUROREl5TXk1cWNHY3wzMjIzMTdmMjJhNjlkMjJjY2Q2Y2UyMmY1NTgxOWRmN2NiZTZjZDRlNGFkMjZlNDg3ODBlNTE4YzFjNjMzMDFm "Coumarin Tyramide 351 [for Tyramide Signal Amplification] (50 tests)")
![Chemical Structure of Coumarin Tyramide 351 [for Tyramide Signal Amplification] (50 tests)](/medias/Tci-440-C4223.jpg?context=bWFzdGVyfHJvb3R8MTQ3NTh8aW1hZ2UvanBlZ3xhRFl3TDJnNU1pODVOalkyTnpBM016VTNOekkyTDFSamFTMDBOREJmUXpReU1qTXVhbkJufDQ4Y2Y2ZTVjMzAxNGJiYzE5YTY0NDIyOTdjNDhjOGYwYmFjZDRmZmNlMzJhYWE4MDhkMTRiZGFkZmNlOWQzMDg "Coumarin Tyramide 351 [for Tyramide Signal Amplification] (50 tests)")
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Purity:
Synonyms:
- 7-Hydroxy-N-(4-hydroxyphenethyl)-2-oxo-2H-chromene-3-carboxamide (50 tests)
Product Documents:
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| 1VIAL |
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Supplemental Product Information:
Step-by-Step Protocol
- Incubate tissue sections as normal, first with primary antibody, followed by HRP-conjugated secondary antibody. (Preliminary testing of primary antibody concentrations between 1:100 and 1:2000 is recommended.)
- Before first use, dissolve a tyramide reagent in 50 µL DMSO. (Aliquot as needed. Store below -20°C. Minimize freeze-thaw cycles.)
- After secondary antibody staining, wash sections with TBST (5 minutes, 3 times).
- Immediately before use, mix the following to prepare the staining solution for each 1 cm2 area to be stained.
- Tyramide reagent solution (from Step 2) 1 µL
- 0.15% H2O2 1 µL
- 1x TBS 98 µL
- Remove slides from TBST and tilt onto a tissue to remove excess buffer. Lay slides flat, immediately apply 100 µL of the staining solution from Step 4 to each section, and allow to react for 1-10 minutes.
- After the reaction, remove excess solution with a tissue and immediately wash with an excess of TBST (5 minutes, 3 times).
- After washing, apply mounting solution and coverslip for observation.
| Product Number | C4223 |
| Molecular Formula / Molecular Weight | C__1__8H__1__5NO__5 = 325.32 |
| Physical State (20 deg.C) | Solid |
Storage Temperature 
|
Frozen (<0°C) |
| Store Under Inert Gas | Store under inert gas |
| Condition to Avoid | Light Sensitive,Air Sensitive,Heat Sensitive |
| CAS RN | 1621579-96-1 |
| Reaxys Registry Number | 27026739 |
Specifications
| Appearance | White to Yellow powder to crystal |
| TSA Immunofluorescence Suitability Test | to pass test |
| Acetonitrile | lt. 2.0 % |
Properties (reference)
GHS
| Pictogram |
|
| Signal Word | Warning |
| Hazard Statements | H371 : May cause damage to organs. |
| Precautionary Statements | P501 : Dispose of contents/ container to an approved waste disposal plant. P260 : Do not breathe dust. P270 : Do not eat, drink or smoke when using this product. P264 : Wash skin thoroughly after handling. P308 + P311 : IF exposed or concerned: Call a POISON CENTER/doctor. P405 : Store locked up. |
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Transport Information:
| H.S.code* | 2932.20-000 |
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