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CAS RN: 65-61-2 | Product Number: A3396
Acridine Orange Solution [for Cell Staining]
![Thumbnail of Acridine Orange Solution [for Cell Staining]](/medias/Tci-98-A3396.jpg?context=bWFzdGVyfHJvb3R8MjczM3xpbWFnZS9qcGVnfGFEWTBMMmcyTWk4NU5UYzVOVEkzTnpNek1qYzRMMVJqYVMwNU9GOUJNek01Tmk1cWNHY3w5YTA0NTA0M2U4NzVkMGMwMzhiNmQ5NTg0MTQ1YjM5YWQ0NjIyMTFlNjg0OTFkMDYwOGJkZjY2ZTg1MDJhYzJl "Acridine Orange Solution [for Cell Staining]")
![Chemical Structure of Acridine Orange Solution [for Cell Staining]](/medias/Tci-440-A3396.jpg?context=bWFzdGVyfHJvb3R8MTg1OTF8aW1hZ2UvanBlZ3xhRGMxTDJnMk5TODVOVGM1TlRJM05qWTNOelF5TDFSamFTMDBOREJmUVRNek9UWXVhbkJufGYyZDdjZjcwZDBlOGFhMzAyMTYzN2RjZDRhODM5NTRkYzRkMDk1NDZkZDkxNzNhMzE0Y2ZjNDRjNGI3Y2Y5YTA "Acridine Orange Solution [for Cell Staining]")
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![Acridine Orange Solution [for Cell Staining]](/_ui/responsive/theme-tci/images/missing_product_zoom.webp "Acridine Orange Solution [for Cell Staining]")
Purity:
Synonyms:
Product Documents:
| Size | Unit Price | Belgium | Japan* | Quantity |
Shipping Information
|
|---|---|---|---|---|---|
| 5ML |
€45.00
|
1 | ≥40 |
|
* Stock available in Belgium: Shipment on the same day
* Stock available in Japan: Please check the Shipping Simulation for estimated shipments. (excludes regulated items and dry ice shipments)
Supplemental Product Information:
Product Information
Volume : 5 mL
Product Form : 0.05 mg/mL solution
Description
Acridine orange is a cell membrane-permeant nucleic acid cell stain, which is incorporated into double-stranded DNA at a ratio of one to three base pairs. It fluoresces at 520nm (Ex. 500nm) when bound to double stranded DNA, and 650nm (Ex. 460nm) when bound to RNA or single-stranded DNA. As acridine orange is mutagenic, this product is supplied as a pre-made solution to prevent exposure during weighing.
Direction for Use
Adjust volume and staining time as necessary. Because some cells need to be fixed prior to staining, preliminary testing should be performed.
1. Remove medium from cells and wash twice with cold PBS(-).
2. Remove PBS(-) and add an appropriate volume of 1:50 Acridine Orange (Product No. A3396) in PBS(-). Allow to stain for 15 minutes.
3. Remove the staining solution and wash twice with PBS(-).
4. Observe cells with a fluorescence microscope while submerged in PBS(-).
Storage
Upon receipt the product should be stored at 4 °C, protected from light, and used within 6 months.
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| Product Number | A3396 |
| Molecular Formula / Molecular Weight | C__1__7H__1__9N__3·HCl = 301.82 |
| Physical State (20 deg.C) | Liquid |
Storage Temperature 
|
Refrigerated (0-10°C) |
| Condition to Avoid | Light Sensitive,Heat Sensitive |
| CAS RN | 65-61-2 |
| PubChem Substance ID | 468590276 |
| MDL Number | MFCD00012660 |
Specifications
| Appearance | Light yellow to Brown clear liquid |
| DNA staining test | to pass test |
Properties (reference)
GHS
Related Laws:
| EC Number | 200-614-0 |
| RTECS# | AR7601000 |
Transport Information:
| HS Number | 3204130090 |
Application
Cell Staining
References
- The frequencies of micronuclei induced by cisplatin in newborn rat astrocytes are increased by 50-Hz, 7.5- and 10-mT electromagnetic fields
- Nucleolus-like bodies of fully-grown mouse oocytes contain key nucleolar proteins but are impoverished for rRNA
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