Published TCIMAIL newest issue No.201
Maximum quantity allowed is 999
Please select the quantity
CAS RN: | Product Number: Y0021
E.coli / Yeast Protein Extraction Buffer
×
Purity:
Synonyms:
Product Documents:
| Size | Unit Price | Belgium | Japan* | Quantity |
Shipping Information
|
|---|---|---|---|---|---|
| 100ML |
€69.00
|
1 | 29 |
|
* Stock available in Belgium: Shipment on the same day
* Stock available in Japan: Please check the Shipping Simulation for estimated shipments. (excludes regulated items and dry ice shipments)
Supplemental Product Information:
Product Information
- Volume : 100 mL
- Additives : WITHOUT protease inhibitors
Description
- This product is supplied as a ready-to-use solution to lyse cultured E. coli / yeast cells. Extracted protein can be used in downstream applications such as Western blotting and protein quantification. This product does not include protease inhibitors.
Storage
- Store at 4 °C.
Directions for Use: Lysis of E. coli cells
-
- Centrifuge E. coli cells at 5000 × g for 10 minutes.
- Remove as much medium as possible from pellet.
- Resuspend pellet in E.coli / Yeast Protein Extraction Buffer (Product No. Y0021). Use 5-10 mL buffer per gram pellet.
- Incubate for 10 minutes at room temperature.
- Centrifuge lysate at 15,000 × g for 10 minutes.
- Transfer the supernatant, containing extracted proteins, to a new tube for downstream applications.
Directions for Use: Lysis of yeast cells
-
- Centrifuge yeast cells at 3000 × g for 10 minutes.
- Remove as much medium as possible from pellet.
- Resuspend pellet in E.coli / Yeast Protein Extraction Buffer (Product No. Y0021). Use 2-4 mL buffer per gram pellet.
- Agitate at room temperature for 10 minutes.
- Centrifuge lysate at 14,000 × g for 10 minutes.
- Transfer the supernatant, containing extracted proteins, to a new tube for downstream applications.
Related Products
- A2215 AEBSF [for Biochemical Research]
- D3789 Disodium Dihydrogen Ethylenediaminetetraacetate Dihydrate [for Biochemical Research]
- E1337 E-64d [for Biochemical Research]
- P2575 Pyrogallol Red (Ready-to-use solution) [for Protein determination]
- P2949 100×Protease Inhibitor Cocktail (EDTA free)
- P2976 100×Protease Inhibitor Cocktail
| Product Number | Y0021 |
| Physical State (20 deg.C) | Liquid |
Storage Temperature 
|
Refrigerated (0-10°C) |
| Condition to Avoid | Heat Sensitive |
Specifications
| Appearance | Colorless to Almost colorlessclear liquid |
| Suitability for protein-analysis (E.coli) | to pass test |
| Suitability for protein-analysis (Yeast) | to pass test |
Properties (reference)
| Specific Gravity (20/20) | 1.06 |
GHS
Related Laws:
Transport Information:
| HS Number | 3822190090 |
Application
E.coli / Yeast Protein Extraction
References
- Non-detergent sulphobetaines: a new class of mild solubilization agents for protein purification
Documents
Product Articles
[Featured Products] Ready-to-use Solutions for Protein Extraction[TCIMAIL No.188] Ready-to-Use Solution for Protein Extraction from E. coli / Yeast Cells
Product Documents (Note: Some products will not have analytical charts available.)
Enter TCI Product Number
Enter KISHIDA Product Number
4-5 alphanumeric characters before the hyphen
7 alphanumeric characters
Safety Data Sheet (SDS)
Please select Language.
The requested SDS is not available.
Please Contact Us for more information.
• The requested SDS is not available.
Specifications
The requested specifications are not available.
Please Contact Us for more information.
The requested specifications are not available.
C of A & Other Certificates
Please enter Lot Number
Incorrect Lot Number. Please input only the 4-5 alphanumeric characters before the hyphen.
Incorrect Lot Number. Please input only the 7 alphanumeric characters.
• Please check that the lot number you have entered is correct. It is 4-5 alphanumeric characters before the hyphen. In particular, please check O (letter) or 0 (number) and I (letter) or 1 (number).
• If your information is correct and you are still not able to view the requested certificates, Please Contact Us for more information.
• Please check that the lot number you have entered is correct. It is 7 alphanumeric characters. In particular, please check O (letter) or 0 (number) and I (letter) or 1 (number).
Sample C of A
This is a sample C of A and may not represent a recently manufactured lot of the product.
A sample C of A for this product is not available at this time.
Analytical Charts
Please enter Lot Number
Incorrect Lot Number. Please input only the 4-5 alphanumeric characters before the hyphen.
Incorrect Lot Number. Please input only the 7 alphanumeric characters.
The requested analytical chart is not available. Sorry for the inconvenience.