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CAS RN: 41085-99-8 | Product Number: D6202
DiI Solution [for Cell Membrane Staining]
![Thumbnail of DiI Solution [for Cell Membrane Staining]](/medias/Tci-98-D6202.jpg?context=bWFzdGVyfHJvb3R8MzgyMXxpbWFnZS9qcGVnfGFHRmpMMmcyTWk4NU5EWTROVFEyTVRVME5USTJMMVJqYVMwNU9GOUVOakl3TWk1cWNHY3wwNjNhNzY3YTk0MmU0ODRkMjVjMjA0ZjM0MDlkM2EwZWIzYjg3MzEwMDMxZDVhZmIzZWE5ZDA4MmVlNTJkNzBj "DiI Solution [for Cell Membrane Staining]")
![Thumbnail of DiI Solution [for Cell Membrane Staining]](/medias/Tci-98-D6202-HeLa-cells-stained-by-DiI-Solution.jpg?context=bWFzdGVyfHBkcC1hcHBsaWNhdGlvbi1pbWFnZXN8MjIzMTh8aW1hZ2UvanBlZ3xhREl5TDJoaE1DODVOVEl4TXpRNU5EVTVPVGs0TDFSamFTMDVPRjlFTmpJd01pMUlaVXhoTFdObGJHeHpMWE4wWVdsdVpXUXRZbmt0UkdsSkxWTnZiSFYwYVc5dUxtcHdad3xlM2ViN2UwYmQ3OGQyMGRmNjczNjE4NWViZTk5MzdmYjBhZmM1MWJiNjk1NGE4MjJlZjJkODJjMjI0ODNiNTQz "DiI Solution [for Cell Membrane Staining]")
![Chemical Structure of DiI Solution [for Cell Membrane Staining]](/medias/Tci-440-D6202.jpg?context=bWFzdGVyfHJvb3R8Mjk1MTF8aW1hZ2UvanBlZ3xhR0prTDJnMk5TODVORFk0TlRRMk1EZzRPVGt3TDFSamFTMDBOREJmUkRZeU1ESXVhbkJufDc1Y2NkMjM1NDZiN2E1ZTNjYjQyNDAzYTRjOTU3Mzk2YWE0NjFkZDUxMjYwNTFhMjlhMDA5OTU1NGQwMDkxNmU "DiI Solution [for Cell Membrane Staining]")
![Chemical Structure of DiI Solution [for Cell Membrane Staining]](/medias/Tci-440-D6202-HeLa-cells-stained-by-DiI-Solution.jpg?context=bWFzdGVyfHBkcC1hcHBsaWNhdGlvbi1pbWFnZXN8MjI4MDExfGltYWdlL2pwZWd8YURJMEwyZzVaQzg1TlRJeE16UTVNemswTkRZeUwxUmphUzAwTkRCZlJEWXlNREl0U0dWTVlTMWpaV3hzY3kxemRHRnBibVZrTFdKNUxVUnBTUzFUYjJ4MWRHbHZiaTVxY0djfGM0ZDY0OTNlMzUxZDdiMWMxOTY4MzU1OWFjMWM2ZWJjOTk5MjFjODUyYTIwMDM5YjhiNTU0NTNjMWE5ZTJmMjQ "DiI Solution [for Cell Membrane Staining]")
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Purity:
Synonyms:
Product Documents:
| Size | Unit Price | Belgium | Japan* | Quantity |
Shipping Information
|
|---|---|---|---|---|---|
| 1ML |
€179.00
|
1 | 17 |
|
* Stock available in Belgium: Shipment on the same day
* Stock available in Japan: Please check the Shipping Simulation for estimated shipments. (excludes regulated items and dry ice shipments)
Supplemental Product Information:
Product Information
- Concentration :1 mM
- Excitation / Emission : 553 nm / 575 nm
Description
- DiI is a lipophilic carbocyanine dye used to label cell membranes. It emits strong fluorescence when its long hydrocarbon chains are incorporated into a lipid bilayer. This product is a ready-to-use solution of DiI which can be used as a tracer in cell fusion, cell adhesion and chemotaxis.
Storage
- Store at room temperature (below 15 °C is recommended) and protect from light.
Directions for Use: Staining live cells
-
- Dilute DiI solution to 5 μM with culture medium.
- Remove medium from cells and replace with 5 μM DiI-containing medium.
- Incubate cells at 37 °C for 10 minutes.
- Remove medium and wash cells 3 times with PBS.
- Observe stained cell membranes via a fluorescence microscope.
Directions for Use: Staining fixed cells
-
- Remove culture medium and wash cells twice with PBS.
- Add 4% paraformaldehyde to cells and incubate at room temperature for 10 minutes.
- Dilute DiI solution to 5 μM with PBS.
- Remove 4% paraformaldehyde and add 5 μM DiI-containing PBS to cells.
- Incubate cells at 37 °C for 10 minutes.
- Remove medium and wash cells 3 times with PBS.
- Observe stained cell membranes via a fluorescence microscope.
Related Products
- D6263 DiD Solution [for Cell Membrane Staining]
- D6539 DiO Solution [for Cell Membrane Staining]
- D6540 DiR Solution [for Cell Membrane Staining]
- P3316 PKH 26 Solution [for Cell Membrane Staining]
| Product Number | D6202 |
| Physical State (20 deg.C) | Liquid |
Storage Temperature 
|
Room Temperature (Recommended in a cool and dark place, <15°C) |
| Condition to Avoid | Light Sensitive |
| CAS RN | 41085-99-8 |
Specifications
| Appearance | Light red to Red clear liquid |
| Cell staining test | to pass test |
Properties (reference)
| Specific Gravity (20/20) | 1.04 |
GHS
| Pictogram |
|
| Signal Word | Danger |
| Hazard Statements | H319 : Causes serious eye irritation. H360 : May damage fertility or the unborn child. H372 : Causes damage to organs through prolonged or repeated exposure. H373 : May cause damage to organs through prolonged or repeated exposure. H350 : May cause cancer. H335 : May cause respiratory irritation. H336 : May cause drowsiness or dizziness. H225 : Highly flammable liquid and vapour. |
| Precautionary Statements | P260 : Do not breathe mist or vapours. P210 : Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking. P201 : Obtain special instructions before use. P280 : Wear protective gloves/ protective clothing/ eye protection/ face protection/ hearing protection. P370 + P378 : In case of fire: Use dry sand, dry chemical or alcohol-resistant foam to extinguish. P308 + P313 : IF exposed or concerned: Get medical advice/ attention. |
Related Laws:
Transport Information:
| UN Number | UN1170 |
| Class | 3 |
| Packing Group | II |
| HS Number | 3822190090 |
Documents
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Other Documents
Related Products
DiI
| Product # | D6452 |
| CAS RN | 41085-99-8 |
| Purity / Analysis Method: | >93.0%(HPLC)(qNMR) |