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CAS RN: 127274-91-3 | Product Number: D6263
DiD Solution [for Cell Membrane Staining]
![Thumbnail of DiD Solution [for Cell Membrane Staining]](/medias/Tci-98-D6263.jpg?context=bWFzdGVyfHJvb3R8MjY0N3xpbWFnZS9qcGVnfGFEZGxMMmhpTVM4NU5UQTRNamMxTmpFM09ESXlMMVJqYVMwNU9GOUVOakkyTXk1cWNHY3xkMzRmOGNjM2E1MTY5ZjFiODEyMzdmY2U3Nzc2YTFkYjU5YzE3NTdmM2Q3MWU2NDI1ODc2Mjk4NGQxODFhOTE5 "DiD Solution [for Cell Membrane Staining]")
![Chemical Structure of DiD Solution [for Cell Membrane Staining]](/medias/Tci-440-D6263.jpg?context=bWFzdGVyfHJvb3R8MjEyNjh8aW1hZ2UvanBlZ3xhREkxTDJoaU5DODVOVEE0TWpjMU5UZzFNRFUwTDFSamFTMDBOREJmUkRZeU5qTXVhbkJufGRmNzMwOWQ0MmY3MzBlYjM4YThkNGNlYzdkM2M3MWE1MmI3M2U1MTQ5YzcyODMxNzkwMTJiMWU1NGFkY2QzZDY "DiD Solution [for Cell Membrane Staining]")
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![DiD Solution [for Cell Membrane Staining]](/_ui/responsive/theme-tci/images/missing_product_zoom.webp "DiD Solution [for Cell Membrane Staining]")
Purity:
Synonyms:
- 2-[5-(3,3-Dimethyl-1-octadecylindolin-2-ylidene)penta-1,3-dien-1-yl]-3,3-dimethyl-1-octadecyl-3H-indol-1-ium Perchlorate Solution
Product Documents:
| Size | Unit Price | Belgium | Japan* | Quantity |
Shipping Information
|
|---|---|---|---|---|---|
| 1ML |
€179.00
|
Contact Us | 19 |
|
* Stock available in Belgium: Shipment on the same day
* Stock available in Japan: Please check the Shipping Simulation for estimated shipments. (excludes regulated items and dry ice shipments)
Supplemental Product Information:
Product Information
- Concentration : 1 mM
- Excitation / Emission : 657 nm / 678 nm
Description
- DiD is a lipophilic carbocyanine dye used to label cell membranes. It emits strong fluorescence when its long hydrocarbon chains are incorporated into a lipid bilayer. This product is a ready-to-use solution of DiD which can be used as a tracer in cell fusion, cell adhesion and chemotaxis.
Storage
- Store at room temperature and protect from light.
Direction for Use
-
Staining live cells
- Dilute DiD solution to 5 μM with culture medium.
- Remove medium from cells and replace with 5 μM DiD-containing medium.
- Incubate cells at 37 °C for 10 minutes.
- Remove medium and wash cells 3 times with PBS.
- Observe stained cell membranes via a fluorescence microscope.
-
Staining fixed cells
- Remove culture medium and wash cells twice with PBS.
- Add 4% paraformaldehyde to cells and incubate at room temperature for 10 minutes.
- Dilute DiD solution to 5 μM with PBS.
- Remove 4% paraformaldehyde and add 5 μM DiD-containing PBS to cells.
- Incubate cells at 37 °C for 10 minutes.
- Remove medium and wash cells 3 times with PBS.
- Observe stained cell membranes via a fluorescence microscope.
Related Products
- D6202 DiI Solution [for Cell Membrane Staining]
- D6539 DiO Solution [for Cell Membrane Staining]
- D6540 DiR Solution [for Cell Membrane Staining]
- P3316 PKH 26 Solution [for Cell Membrane Staining]
| Product Number | D6263 |
| Molecular Formula / Molecular Weight | C__6__1H__9__9ClN__2O__4 = 959.92 |
| Physical State (20 deg.C) | Liquid |
Storage Temperature 
|
Room Temperature (Recommended in a cool and dark place, <15°C) |
| CAS RN | 127274-91-3 |
Specifications
| Appearance | Blue to Dark blue clear liquid |
| Cell staining test | to pass test |
Properties (reference)
GHS
| Pictogram |
|
| Signal Word | Danger |
| Hazard Statements | H319 : Causes serious eye irritation. H360 : May damage fertility or the unborn child. H372 : Causes damage to organs through prolonged or repeated exposure. H373 : May cause damage to organs through prolonged or repeated exposure. H350 : May cause cancer. H225 : Highly flammable liquid and vapour. |
| Precautionary Statements | P260 : Do not breathe mist or vapours. P210 : Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking. P201 : Obtain special instructions before use. P280 : Wear protective gloves/ protective clothing/ eye protection/ face protection/ hearing protection. P370 + P378 : In case of fire: Use dry sand, dry chemical or alcohol-resistant foam to extinguish. P308 + P313 : IF exposed or concerned: Get medical advice/ attention. |
Related Laws:
Transport Information:
| UN Number | UN1993 |
| Class | 3 |
| Packing Group | II |
| HS Number | 2208909190 |
Documents
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Other Documents
Related Products
DiD
| Product # | D6451 |
| CAS RN | 127274-91-3 |
| Purity / Analysis Method: | >95.0%(HPLC)(qNMR) |