CAS RN: | Product Number: W0024

Western Blot Stripping Buffer [for Biochemical Research]

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Size	Unit Price	Belgium	Japan*	Quantity	Shipping Information
250ML	46,00 €	1	35		Shipping Simulation

* Stock available in Belgium: Shipment on the same day
* Stock available in Japan: Please check the Shipping Simulation for estimated shipments. (excludes regulated items and dry ice shipments)

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Product Information

Volume : 250 mL

Description

Western Blot Stripping Buffer is a reagent used for removing antibodies from membranes that detects chemiluminescence in Western blotting. After chemiluminescent detection, membranes treated with Stripping Buffer can be reused for antibody concentration studies or for re-detection with a different primary antibody. The Stripping Buffer is useful for saving samples and shortening the experiment time.
Note: Dissolve the precipitation at 25-30 °C, if precipitation occurs during storage. The Stripping Buffer is acidic and corrosive. Please wear protective equipment when using it. Metal tweezers and trays should not be used with the Stripping Buffer.

Storage

Store at room temperature.

Direction for Use

1. After detecting chemiluminescence, wash the membrane twice for 10 minutes with TBS-T or PBS-T buffer.
2. Incubate the membrane with the Stripping Buffer for 30 minutes at room temperature.
  Note: Depending on the affinity between the antigen and the antibody, the antibody may not be completely removed from the membrane.Perform blocking and antigen-antibody reaction again.
3. Use a sufficient volume of the Stripping Buffer to ensure that the membrane is completely submerged.
4. Wash the membrane three times for 10 minutes each with TBS-T or PBS-T buffer.

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General Information plus

Specifications & Properties plus

Specifications

Appearance	Colorless to Almost colorlessclear liquid
Functionality test	to pass test

Properties (reference)

Specific Gravity (20/20)

1.00

Safety & Regulations plus

GHS

Pictogram
Signal Word	Warning
Hazard Statements	H319 : Causes serious eye irritation. H371 : May cause damage to organs. H373 : May cause damage to organs through prolonged or repeated exposure.
Precautionary Statements	P501 : Dispose of contents/ container to an approved waste disposal plant. P260 : Do not breathe mist or vapours. P264 : Wash skin thoroughly after handling. P280 : Wear eye protection/ face protection. P337 + P313 : If eye irritation persists: Get medical advice/ attention. P308 + P311 : IF exposed or concerned: Call a POISON CENTER/doctor.