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CAS RN: | Product Number: A2509

Anti-Sialyl Lewis A Monoclonal Antibody (2D3)

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Product Information
Volume/Quantity: 0.1 mg/vial
Product Form: Purified mouse monoclonal antibody/IgM, κ-chain
Buffer: Phosphate buffered-saline(-) ((PBS(-))
Preparation: The antibody is purified from ascites fluid.
Preservative: 0.05% sodium azide
Stabilizer: None
Immunogen: Immunized with human colon cancer cells (Colo201), and screened using sialyl Lewis A-active glycolipids
Specificity: Sialyl Lewis A antigen
Clone Name: 2D3

Immunohistochemistry, flow-cytometric analysis, ELISA, TLC-immunostaining, Western blotting, cell adhesion inhibition experiments.

Store at -20 ºC to -80 ºC (Preferably at -80 ºC). It is not recommended storing at 4 ºC. Avoid repeated freezing and thawing.

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Product Number A2509
Physical State (20 deg.C) Liquid
Storage Temperature -80°C
Condition to Avoid Heat Sensitive
Appearance Colorless to Almost colorless clear liquid
Titer(ELISA) to pass test
Properties (reference)
Related Laws:
Transport Information:
HS Number 3002150000
Anti-Sialyl Lewis A Monoclonal Antibody (2D3)

Adhesion of human cancer cells to vascular endothelium mediated by a carbohydrate antigen, sialyl Lewis A

A. Takada, K. Ohmori, N. Takahashi, K. Tsuyuoka, A. Yago, K. Zenita, A. Hasegawa, R. Kannagi, Biochem. Biophys. Res. Commun. 1991, 179, 713-719.

Northern hybridization analysis of VH gene expression in murine monoclonal antibodies directed to cancer-associated ganglioside antigens having various sialic acid linkages

K. Zenita, K. Hirashima, K. Shigeta, N. Hiraiwa, A. Takada, K. Hashimoto, E. Fujimoto, K. Yago, R. Kannagi, J. Immunol. 1990, 144, 4442-4451.

Contribution of carbohydrate antigens sialyl Lewis A and sialyl Lewis X to adhesion of human cancer cells to vascular endothelium

A. Takada, K. Ohmori, T. Yoneda, K. Tsuyuoka, A. Hasegawa, M. Kiso, R. Kannagi, Cancer Res. 1993, 53, 354-361.

Importance of E-selectin (ELAM-1) and sialyl Lewisa in the adhesion of pancreatic carcinoma cells to activated endothelium

K. Iwai, H. Ishikura, M. Kaji, H. Sugiura, A. Ishizu, C. Takahashi, H. Kato, T. Tanabe, T. Yoshiki, Int. J. Cancer 1993, 54, 972-977.

Hypoxia induces adhesion molecules on cancer cells: A missing link between Warburg effect and induction of selectin-ligand carbohydrates

T. Koike, N. Kimura, K. Miyazaki, T. Yabuta, K. Kumamoto, S. Takenoshita, J. Chen, M. Kobayashi, M. Hosokawa, A. Taniguchi, T. Kojima, N. Ishida, M. Kawakita, H. Yamamoto, H. Takematsu, A. Suzuki, Y. Kozutsumi, R. Kannagi, Proc. Natl. Acad. Sci. U.S.A. 2004, 101, 8132-8137.

Clinical application of functional glycoproteomics - dissection of glycotopes carried by soluble CD44 variantsin sera of patients with cancers

K.-T. Lim, K. Miyazaki, N. Kimura, M. Izawa, R. Kannagi, Proteomics 2008, 8, 3263-3273.

Colonic epithelial cells express specific ligands for mucosal macrophage immunosuppressive receptors, siglec-7 and -9

K. Miyazaki, K. Sakuma, Y. I. Kawamura, M. Izawa, K. Ohmori, M. Mitsuki, T. Yamaji, Y. Hashimoto, A. Suzuki, Y. Saito, T. Dohi, R. Kannagi, J. Immunol. 2012, 188, 4690-4700.

Peritoneal colonization by human pancreatic cancer cells is inhibited by antisense FUT3 sequence

M. Aubert, L. Panicot-Dubois, C. Crotte, V. Sbarra, D. Lombardo, M.-O. Sadoulet, E. Mas, Int. J. Cancer 2000, 88, 558-565.

Detection of ligands for selectins in the oligosaccharide fraction of human milk

S. Rudloff, C. Stefan, G. Pohlentz, C. Kunz, Eur. J. Nutr. 2002, 41, 85-92.

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