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CAS RN: | Product Number: C3488
Coomassie Brilliant Blue G-250 (Ready-to-use solution) [for Electrophoresis]
*Stock available in Belgium will be delivered in 1 to 3 days
*Stock available in Japan will be delivered in 1 to 2 weeks (excludes regulated items and dry ice shipments).
Volume : 500 mL
This product is supplied as a ready-to-use solution containing Coomassie Brilliant Blue G-250 (CBB G-250) for gel staining after electrophoresis. No hazardous methanol or acetic acid is in the formulation of this product.
Direction for Use
1 : After electrophoresis, wash the gel in a tray with 50 ∼ 100 mL deionized water for 5 minutes three times.
2 : Gently mix the CBB solution bottle (Product No. C3488).
3 : Remove the water used for washing, add 25 ∼ 50 mL CBB solution (Product No. C3488) into the tray, and let the gel stain for 1 hour while shaking gently at room temperature.
4 : Destain the gel in 50 ∼ 100 mL deionized water for 1 hour.
5 : If the background is high, destain the gel in 50 ∼ 100 mL deionized water for more than 1 hour or overnight at room temperature.
Keep the product away from light and store at room temperature. The product should be used within 1 year from purchase.
[For gel preparing]
A1132 Acrylamide Monomer [for Electrophoresis]
A2098 Ammonium Peroxodisulfate [for Electrophoresis]
D1820 Sodium Deoxycholate [for Electrophoresis]
D3647 DL-Dithiothreitol [for Electrophoresis]
G0316 Glycerol [for Electrophoresis]
G0317 Glycine [for Electrophoresis]
M1948 2-Mercaptoethanol [for Electrophoresis]
M0506 N,N'-Methylenebisacrylamide [for Electrophoresis]
S0588 Sodium Dodecyl Sulfate [for Electrophoresis]
T2515 N,N,N',N'-Tetramethylethylenediamine [for Electrophoresis]
T2516 Tris(hydroxymethyl)aminomethane [for Electrophoresis]
B3193 Coomassie Brilliant Blue G-250 [for Electrophoresis]
B3194 Coomassie Brilliant Blue R-250 [for Electrophoresis]
F0718 Fast Green FCF [for Biochemical Research]
|Physical State (20 deg.C)||Liquid|
|Appearance||Green to Dark green to Dark blue clear liquid|
|Protein staining test||to pass test|
|Hazard Statements||H315 : Causes skin irritation.
H319 : Causes serious eye irritation.
H360 : May damage fertility or the unborn child.
H372 : Causes damage to organs through prolonged or repeated exposure.
H373 : May cause damage to organs through prolonged or repeated exposure.
H370 : Causes damage to organs.
H340 : May cause genetic defects.
H225 : Highly flammable liquid and vapour.
|Precautionary Statements||P260 : Do not breathe dust/ fume/ gas/ mist/ vapours/ spray.
P210 : Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
P201 : Obtain special instructions before use.
P280 : Wear protective gloves/ protective clothing/ eye protection/ face protection.
P370 + P378 : In case of fire: Use dry sand, dry chemical or alcohol-resistant foam to extinguish.
P308 + P311 : IF exposed or concerned: Call a POISON CENTER/doctor.
- Environmentally benign staining procedure for electrophoresis gels using Coomassie Brilliant Blue.
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